Single-cell, locus-specific bisulfite sequencing (SLBS) for direct detection of epimutations in DNA methylation patterns.
| Autor: | Gravina S; Department of Genetics, Albert Einstein College of Medicine, Bronx, NY 10461, USA silvia.gravina@einstein.yu.edu., Ganapathi S; Department of Genetics, Albert Einstein College of Medicine, Bronx, NY 10461, USA., Vijg J; Department of Genetics, Albert Einstein College of Medicine, Bronx, NY 10461, USA silvia.gravina@einstein.yu.edu. |
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| Jazyk: | angličtina |
| Zdroj: | Nucleic acids research [Nucleic Acids Res] 2015 Aug 18; Vol. 43 (14), pp. e93. Date of Electronic Publication: 2015 Apr 19. |
| DOI: | 10.1093/nar/gkv366 |
| Abstrakt: | Stochastic epigenetic changes drive biological processes, such as development, aging and disease. Yet, epigenetic information is typically collected from millions of cells, thereby precluding a more precise understanding of cell-to-cell variability and the pathogenic history of epimutations. Here we present a novel procedure for directly detecting epimutations in DNA methylation patterns using single-cell, locus-specific bisulfite sequencing (SLBS). We show that within gene promoter regions of mouse hepatocytes the epimutation rate is two orders of magnitude higher than the mutation rate. (© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.) |
| Databáze: | MEDLINE |
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