Inhibitory effects of emodin, baicalin, schizandrin and berberine on hefA gene: treatment of Helicobacter pylori-induced multidrug resistance.

Autor:	Huang YQ; Yan-Qiang Huang, Gan-Rong Huang, Hua-Ying Tang, Xiao-Qiang Mo, Bing-Pu Chen, Li-Juan Zhao, Xiao-Feng Huang, Hong-Yu Wei, Lian-Deng Wei, Department of Medical Microbiology and Immunology, Youjiang Medical College for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China., Huang GR; Yan-Qiang Huang, Gan-Rong Huang, Hua-Ying Tang, Xiao-Qiang Mo, Bing-Pu Chen, Li-Juan Zhao, Xiao-Feng Huang, Hong-Yu Wei, Lian-Deng Wei, Department of Medical Microbiology and Immunology, Youjiang Medical College for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China., Wu MH; Yan-Qiang Huang, Gan-Rong Huang, Hua-Ying Tang, Xiao-Qiang Mo, Bing-Pu Chen, Li-Juan Zhao, Xiao-Feng Huang, Hong-Yu Wei, Lian-Deng Wei, Department of Medical Microbiology and Immunology, Youjiang Medical College for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China., Tang HY; Yan-Qiang Huang, Gan-Rong Huang, Hua-Ying Tang, Xiao-Qiang Mo, Bing-Pu Chen, Li-Juan Zhao, Xiao-Feng Huang, Hong-Yu Wei, Lian-Deng Wei, Department of Medical Microbiology and Immunology, Youjiang Medical College for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China., Huang ZS; Yan-Qiang Huang, Gan-Rong Huang, Hua-Ying Tang, Xiao-Qiang Mo, Bing-Pu Chen, Li-Juan Zhao, Xiao-Feng Huang, Hong-Yu Wei, Lian-Deng Wei, Department of Medical Microbiology and Immunology, Youjiang Medical College for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China., Zhou XH; Yan-Qiang Huang, Gan-Rong Huang, Hua-Ying Tang, Xiao-Qiang Mo, Bing-Pu Chen, Li-Juan Zhao, Xiao-Feng Huang, Hong-Yu Wei, Lian-Deng Wei, Department of Medical Microbiology and Immunology, Youjiang Medical College for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China., Yu WQ; Yan-Qiang Huang, Gan-Rong Huang, Hua-Ying Tang, Xiao-Qiang Mo, Bing-Pu Chen, Li-Juan Zhao, Xiao-Feng Huang, Hong-Yu Wei, Lian-Deng Wei, Department of Medical Microbiology and Immunology, Youjiang Medical College for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China., Su JW; Yan-Qiang Huang, Gan-Rong Huang, Hua-Ying Tang, Xiao-Qiang Mo, Bing-Pu Chen, Li-Juan Zhao, Xiao-Feng Huang, Hong-Yu Wei, Lian-Deng Wei, Department of Medical Microbiology and Immunology, Youjiang Medical College for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China., Mo XQ; Yan-Qiang Huang, Gan-Rong Huang, Hua-Ying Tang, Xiao-Qiang Mo, Bing-Pu Chen, Li-Juan Zhao, Xiao-Feng Huang, Hong-Yu Wei, Lian-Deng Wei, Department of Medical Microbiology and Immunology, Youjiang Medical College for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China., Chen BP; Yan-Qiang Huang, Gan-Rong Huang, Hua-Ying Tang, Xiao-Qiang Mo, Bing-Pu Chen, Li-Juan Zhao, Xiao-Feng Huang, Hong-Yu Wei, Lian-Deng Wei, Department of Medical Microbiology and Immunology, Youjiang Medical College for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China., Zhao LJ; Yan-Qiang Huang, Gan-Rong Huang, Hua-Ying Tang, Xiao-Qiang Mo, Bing-Pu Chen, Li-Juan Zhao, Xiao-Feng Huang, Hong-Yu Wei, Lian-Deng Wei, Department of Medical Microbiology and Immunology, Youjiang Medical College for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China., Huang XF; Yan-Qiang Huang, Gan-Rong Huang, Hua-Ying Tang, Xiao-Qiang Mo, Bing-Pu Chen, Li-Juan Zhao, Xiao-Feng Huang, Hong-Yu Wei, Lian-Deng Wei, Department of Medical Microbiology and Immunology, Youjiang Medical College for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China., Wei HY; Yan-Qiang Huang, Gan-Rong Huang, Hua-Ying Tang, Xiao-Qiang Mo, Bing-Pu Chen, Li-Juan Zhao, Xiao-Feng Huang, Hong-Yu Wei, Lian-Deng Wei, Department of Medical Microbiology and Immunology, Youjiang Medical College for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China., Wei LD; Yan-Qiang Huang, Gan-Rong Huang, Hua-Ying Tang, Xiao-Qiang Mo, Bing-Pu Chen, Li-Juan Zhao, Xiao-Feng Huang, Hong-Yu Wei, Lian-Deng Wei, Department of Medical Microbiology and Immunology, Youjiang Medical College for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China.
Jazyk:	angličtina
Zdroj:	World journal of gastroenterology [World J Gastroenterol] 2015 Apr 14; Vol. 21 (14), pp. 4225-31.
DOI:	10.3748/wjg.v21.i14.4225
Abstrakt:	Aim: To investigate the inhibitory effects of emodin, baicalin, etc. on the hefA gene of multidrug resistance (MDR) in Helicobacter pylori (H. pylori). Methods: The double dilution method was used to screen MDR H. pylori strains and determine the minimum inhibitory concentrations (MICs) of emodin, baicalin, schizandrin, berberine, clarithromycin, metronidazole, tetracycline, amoxicillin and levofloxacin against H. pylori strains. After the screened MDR stains were treated with emodin, baicalin, schizandrin or berberine at a 1/2 MIC concentration for 48 h, changes in MICs of amoxicillin, tetracycline, levofloxacin, metronidazole and clarithromycin were determined. MDR strains with reduced MICs of amoxicillin were selected to detect the hefA mRNA expression by real-time quantitative PCR. Results: A total of four MDR H. pylori strains were screened. Treatment with emodin, baicalin, schizandrin and berberine significantly decreased the MICs of amoxicillin and tetracycline against some strains, decreased by 1 to 2 times, but did not significantly change the MICs of clarithromycin, levofloxacin, and metronidazole against MDR strains. In the majority of strains with reduced MICs of amoxicillin, hefA mRNA expression was decreased; one-way ANOVA (SPSS 12.0) used for comparative analysis, P < 0.05. Conclusion: Emodin, baicalin, schizandrin and berberine significantly decreased the MICs of amoxicillin and tetracycline against some H. pylori strains, possibly by mechanisms associated with decreasing hefA mRNA expression.
Databáze:	MEDLINE
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