A 3' external transcribed spacer in a tRNA transcript acts as a sponge for small RNAs to prevent transcriptional noise.

Autor:	Lalaouna D; Department of Biochemistry, RNA Group, Université de Sherbrooke, Sherbrooke, Québec, J1E 4K8, Canada., Carrier MC; Department of Biochemistry, RNA Group, Université de Sherbrooke, Sherbrooke, Québec, J1E 4K8, Canada., Semsey S; Center for Models of Life, Niels Bohr Institute, University of Copenhagen, DK-2100 Copenhagen, Denmark., Brouard JS; Department of Biochemistry, RNA Group, Université de Sherbrooke, Sherbrooke, Québec, J1E 4K8, Canada., Wang J; Wadsworth Center, New York State Department of Health, Albany, NY 12208, USA., Wade JT; Wadsworth Center, New York State Department of Health, Albany, NY 12208, USA., Massé E; Department of Biochemistry, RNA Group, Université de Sherbrooke, Sherbrooke, Québec, J1E 4K8, Canada. Electronic address: eric.masse@usherbrooke.ca.
Jazyk:	angličtina
Zdroj:	Molecular cell [Mol Cell] 2015 May 07; Vol. 58 (3), pp. 393-405. Date of Electronic Publication: 2015 Apr 16.
DOI:	10.1016/j.molcel.2015.03.013
Abstrakt:	During ribosomal and transfer RNA maturation, external transcribed spacer (ETS) and internal transcribed spacer (ITS) sequences are excised and, as non-functional by-products, are rapidly degraded. However, we report that the 3'ETS of the glyW-cysT-leuZ polycistronic tRNA precursor is highly and specifically enriched by co-purification with at least two different small regulatory RNAs (sRNAs), RyhB and RybB. Both sRNAs are shown to base pair with the same region in the 3'ETS of leuZ (3'ETS(leuZ)). Disrupting the pairing by mutating 3'ETS(leuZ) strongly increased the activity of sRNAs, even under non-inducing conditions. Our results indicate that 3'ETS(leuZ) prevents sRNA-dependent remodeling of tricarboxylic acid (TCA) cycle fluxes and decreases antibiotic sensitivity when sRNAs are transcriptionally repressed. This suggests that 3'ETS(leuZ) functions as a sponge to absorb transcriptional noise from repressed sRNAs. Additional data showing RybB and MicF sRNAs are co-purified with ITS(metZ-metW) and ITS(metW-metV) strongly suggest a wide distribution of this phenomenon. (Copyright © 2015 Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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