Discovery of the 2-phenyl-4,5,6,7-Tetrahydro-1H-indole as a novel anti-hepatitis C virus targeting scaffold.

Autor: Andreev IA; EDASA Scientific Srls., Via Stingi, 37, 66050 San Salvo, CH, Italy; Chemistry Department of Lomonosov Moscow State University, Moscow, 119991, GSP-2, Leninskie Gory, 1/3, Russia., Manvar D; Department of Microbiology, Biochemistry and Molecular Genetics, Rutgers, The State University of New Jersey, New Jersey Medical School, NJ 07103, USA., Barreca ML; Department of Pharmaceutical Sciences, University of Perugia, Via A. Fabretti, 48, 06123 Perugia, Italy. Electronic address: lbarreca@unipg.it., Belov DS; EDASA Scientific Srls., Via Stingi, 37, 66050 San Salvo, CH, Italy; Chemistry Department of Lomonosov Moscow State University, Moscow, 119991, GSP-2, Leninskie Gory, 1/3, Russia., Basu A; Department of Microbiology, Biochemistry and Molecular Genetics, Rutgers, The State University of New Jersey, New Jersey Medical School, NJ 07103, USA., Sweeney NL; Department of Chemistry and Biochemistry, University of Wisconsin-Milwaukee, 3210 N. Cramer St., Milwaukee, WI 53211, USA., Ratmanova NK; Chemistry Department of Lomonosov Moscow State University, Moscow, 119991, GSP-2, Leninskie Gory, 1/3, Russia., Lukyanenko ER; EDASA Scientific Srls., Via Stingi, 37, 66050 San Salvo, CH, Italy., Manfroni G; Department of Pharmaceutical Sciences, University of Perugia, Via A. Fabretti, 48, 06123 Perugia, Italy., Cecchetti V; Department of Pharmaceutical Sciences, University of Perugia, Via A. Fabretti, 48, 06123 Perugia, Italy., Frick DN; Department of Chemistry and Biochemistry, University of Wisconsin-Milwaukee, 3210 N. Cramer St., Milwaukee, WI 53211, USA., Altieri A; EDASA Scientific Srls., Via Stingi, 37, 66050 San Salvo, CH, Italy. Electronic address: aaltieri@edasascientific.com., Kaushik-Basu N; Department of Microbiology, Biochemistry and Molecular Genetics, Rutgers, The State University of New Jersey, New Jersey Medical School, NJ 07103, USA. Electronic address: kaushik@njms.rutgers.edu., Kurkin AV; Chemistry Department of Lomonosov Moscow State University, Moscow, 119991, GSP-2, Leninskie Gory, 1/3, Russia.
Jazyk: angličtina
Zdroj: European journal of medicinal chemistry [Eur J Med Chem] 2015; Vol. 96, pp. 250-8. Date of Electronic Publication: 2015 Apr 10.
DOI: 10.1016/j.ejmech.2015.04.022
Abstrakt: Although all-oral direct-acting antiviral (DAA) therapy for hepatitis C virus (HCV) treatment is now a reality, today's HCV drugs are expensive, and more affordable drugs are still urgently needed. In this work, we report the identification of the 2-phenyl-4,5,6,7-Tetrahydro-1H-indole chemical scaffold that inhibits cellular replication of HCV genotype 1b and 2a subgenomic replicons. The anti-HCV genotype 1b and 2a profiling and effects on cell viability of a selected representative set of derivatives as well as their chemical synthesis are described herein. The most potent compound 39 displayed EC50 values of 7.9 and 2.6 μM in genotype 1b and 2a, respectively. Biochemical assays showed that derivative 39 had no effect on HCV NS5B polymerase, NS3 helicase, IRES mediated translation and selected host factors. Thus, future work will involve both the chemical optimization and target identification of 2-phenyl-4,5,6,7-Tetrahydro-1H-indoles as new anti-HCV agents.
(Copyright © 2015 Elsevier Masson SAS. All rights reserved.)
Databáze: MEDLINE
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