Are allergen batch differences and the use of double skin prick test important?

Autor: Thomsen GF; Department of Occupational Medicine, Hospital of Southwest Denmark, Finsensgade 35, 6700, Esbjerg, Denmark. gert@leifi.dk., Schlünssen V; Department of Public Health, Danish Ramazzini Centre, University of Aarhus, Aarhus, Denmark. vs@ph.au.dk.; Department of Occupational Medicine, Danish Ramazzini centre, Aarhus University Hospital, Aarhus, Denmark. vs@ph.au.dk., Skadhauge LR; Department of Occupational Medicine, Hospital of Southwest Denmark, Finsensgade 35, 6700, Esbjerg, Denmark. lars.rauff.skadhauge@rsyd.dk.; Institute of Regional Health Research, Faculty of Health Sciences, University of Southern Denmark, Odense, Denmark. lars.rauff.skadhauge@rsyd.dk., Malling TH; Department of Occupational Medicine, Danish Ramazzini Centre, Aalborg University Hospital, Aalborg, Denmark. t.malling@rn.dk., Sherson DL; Department of Occupational Medicine, Odense University Hospital, Odense, Denmark. david.sherson@rsyd.dk.; Department of Pulmonal Medicine, Odense University Hospital, Odense, Denmark. david.sherson@rsyd.dk., Omland Ø; Department of Occupational Medicine, Danish Ramazzini Centre, Aalborg University Hospital, Aalborg, Denmark. oo@rn.dk.; Department of Health Science and Technology, The Faculty of Medicine; Aalborg University Hospital, Aalborg, Denmark. oo@rn.dk., Sigsgaard T; Department of Public Health, Danish Ramazzini Centre, University of Aarhus, Aarhus, Denmark. ts@ph.au.dk.
Jazyk: angličtina
Zdroj: BMC pulmonary medicine [BMC Pulm Med] 2015 Apr 09; Vol. 15, pp. 33. Date of Electronic Publication: 2015 Apr 09.
DOI: 10.1186/s12890-015-0021-3
Abstrakt: Background: Skin prick tests (SPT) are widely used both in clinical diagnostics and in research. The standardization of allergen extracts is well documented to be crucial for the validity of SPT, whereas less emphasis has been placed on reproducibility and the SPT procedure itself. The objectives of this study are to clarify how the double skin prick test procedure influence the sensitivity and specificity of the test and to analyse the differences in weal size in skin prick tests between two batches of allergen extracts from the same vendor.
Methods: The association between rhinitis and SPT was assessed among 1135 persons from a general population sample. SPT was performed twice with 10 common aeroallergens. In a subsample of 90 persons SPT was performed simultaneously with five of the allergens using different batches.
Results: Thirty percent had at least one positive SPT. Among asthmatics this number was 62%. Only minor differences were seen between the sizes of two weals from the same batch. A second SPT with the same batch did not change the association between rhinitis and sensitization. When performing SPT with two different batches disagreement was observed in 2% (Birch) to 11% (Cat) of the subjects.
Conclusions: Performing SPT twice with the same allergen batch does not enhance the validity of the test, and value of double testing can be questioned. Considerable differences in SPT response with different batches from the same manufacturer were observed. Thus inter batch differences in allergen extracts might be a source of variability.
Databáze: MEDLINE
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