Analysis of the anti-inflammatory and chemopreventive potential and description of the antimutagenic mode of action of the Annona crassiflora methanolic extract.

Autor: Rocha RS; a Center of Studies in Stem Cells, Cell Therapy and Genetic Toxicology (CeTroGen), University Hospital (NHU), Federal University of Mato Grosso do Sul (UFMS) , Campo Grande , MS , Brazil .; b Center of Biological and Health Sciences (CCBS), Federal University of Mato Grosso do Sul (UFMS) , Campo Grande , MS , Brazil ., Kassuya CA; c Faculty of Health Sciences (FCS), Federal University of Grande Dourados (UFGD) , Dourados , MS , Brazil ., Formagio AS; d Faculty of Agricultural Sciences (FCA), Federal University of Grande Dourados (UFGD) , Dourados , MS , Brazil ., Mauro Mde O; a Center of Studies in Stem Cells, Cell Therapy and Genetic Toxicology (CeTroGen), University Hospital (NHU), Federal University of Mato Grosso do Sul (UFMS) , Campo Grande , MS , Brazil .; e Midwest Pro Network - Graduate Program in Biotechnology and Biodiversity, Federal University of Mato Grosso do Sul (UFMS) , Campo Grande , MS , Brazil ., Andrade-Silva M; c Faculty of Health Sciences (FCS), Federal University of Grande Dourados (UFGD) , Dourados , MS , Brazil ., Monreal AC; b Center of Biological and Health Sciences (CCBS), Federal University of Mato Grosso do Sul (UFMS) , Campo Grande , MS , Brazil ., Cunha-Laura AL; b Center of Biological and Health Sciences (CCBS), Federal University of Mato Grosso do Sul (UFMS) , Campo Grande , MS , Brazil .; f Master's Programme in Pharmacy, Center of Biological and Health Sciences (CCBS), Federal University of Mato Grosso do Sul (UFMS) , Campo Grande , MS , Brazil , and., Vieira Mdo C; d Faculty of Agricultural Sciences (FCA), Federal University of Grande Dourados (UFGD) , Dourados , MS , Brazil ., Oliveira RJ; a Center of Studies in Stem Cells, Cell Therapy and Genetic Toxicology (CeTroGen), University Hospital (NHU), Federal University of Mato Grosso do Sul (UFMS) , Campo Grande , MS , Brazil .; f Master's Programme in Pharmacy, Center of Biological and Health Sciences (CCBS), Federal University of Mato Grosso do Sul (UFMS) , Campo Grande , MS , Brazil , and.; g Graduate Program in Health and Development in the Midwest Region, School of Medicine 'Dr. Hélio Mandetta' (FAMED), Federal University of Mato Grosso do Sul (UFMS) , Campo Grande , MS , Brazil.
Jazyk: angličtina
Zdroj: Pharmaceutical biology [Pharm Biol] 2016; Vol. 54 (1), pp. 35-47. Date of Electronic Publication: 2015 Apr 17.
DOI: 10.3109/13880209.2015.1014567
Abstrakt: Context: Annona crassiflora Mart. (Annonaceae) is a medicinal plant that is widely used in folk medicine, which leads to its investigation as a potential source of new pharmacological principles.
Objective: This study describes the anti-inflammatory, antiallodynic, and antimutagenic/chemopreventive activities of the leaves A. crassiflora methanolic extract. Its antimutagenic mode of action was analyzed in a plant or animal experimental model.
Materials and Methods: Total flavonoids were quantified by spectrophotometry at 415 nm and its composition was analyzed by (1)H NMR spectra. Animals received orally, 30, 100, and 300 mg/kg of extract in both tests, carrageenan-induced paw edema and myeloperoxidase activity. Animals were treated with 100 and 300 mg/kg, in all the analyzed tests, pleural cell migration and protein exudation, carrageenan-induced cell migration into the pouch, induction of joint inflammation and carrageenan-induced allodynia response in the mouse paw. To evaluate the antimutagenic/chemopreventive activity through the Allium cepa test, we used 5, 10, and 15 mg/L of extract, and for the micronucleus test in the peripheral blood, we used the dose of 15 mg/kg.
Results: The fractionation of the ethyl acetate (EA) fraction, resulting from the partition of the methanol extract of the A. crassiflora, afforded through chromatographic methods resulted in the isolation of kaempferol 3-O-β-glucoside and kaempferol 3-O-β-diglucoside. Oral treatment with 100 and 300 mg/kg of extract significantly inhibited the carrageenan-induced edema formation, with inhibitions of 53 ± 7% and 47 ± 10%; in MPO activity, the observed inhibitions were 60 ± 7% for 100 mg/kg treatment and 63 ± 7% for 300 mg/kg. The ACME reduced significantly the total leukocytes (an inhibition of 78 ± 9% with 100 mg/kg and 90 ± 7% with 300 mg/kg) and protein levels (approximately 100% inhibition with both doses) in the pleurisy model. In carrageenan-induced leukocyte migration into the pouch, the extract inhibited leukocyte migration only when administered 300 mg/kg per dose (the reduction was 43 ± 5%). Pretreatment with extract failed to reduce the zymosan-induced edema formation and did not inhibit the carrageenan-induced mechanical allodynia. Damage reduction in Allium cepa tested with different concentrations (5, 10, and 15 mg/L) was 66.17, 75.75, and 69.19% for the pre-treatment; 72.72, 33.33, and 22.22% for the simple simultaneous treatment; 100.50, 93.93, and 102.52% for the simultaneous treatment with pre-incubation; 89.39, 79.79, and 84.34%; for the post-treatment, and 86.36, 81.31, and 93.43% for the continuous treatment. The antimutagenic evaluation in the micronucleous test showed a damage reduction of 75.00 and 64.58% for the pre-treatment and simultaneous protocols, respectively. The post-treatment protocol increased the cyclophosphamide effects in 45.83%.
Conclusion: These results suggest that this medicinal plant has chemopreventive and anti-inflammatory therapeutic potential.
Databáze: MEDLINE
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