In situ expression of eukaryotic ice-binding proteins in microbial communities of Arctic and Antarctic sea ice.

Autor:	Uhlig C; Polar Biological Oceanography, Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research, Am Handelshafen 12, Bremerhaven, Germany.; Marine Geochemistry, Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research, Am Handelshafen 12, Bremerhaven, Germany., Kilpert F; Computing Centre, Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research, Am Handelshafen 12, Bremerhaven, Germany.; Hochschule Bremerhaven, Department of Technology, An der Karlstadt 8, Bremerhaven, Germany., Frickenhaus S; Computing Centre, Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research, Am Handelshafen 12, Bremerhaven, Germany.; Hochschule Bremerhaven, Department of Technology, An der Karlstadt 8, Bremerhaven, Germany., Kegel JU; Ecological Chemistry, Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research, Am Handelshafen 12, Bremerhaven, Germany., Krell A; Polar Biological Oceanography, Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research, Am Handelshafen 12, Bremerhaven, Germany., Mock T; School of Environmental Sciences, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, Norfolk, UK., Valentin K; Polar Biological Oceanography, Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research, Am Handelshafen 12, Bremerhaven, Germany., Beszteri B; Polar Biological Oceanography, Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research, Am Handelshafen 12, Bremerhaven, Germany.
Jazyk:	angličtina
Zdroj:	The ISME journal [ISME J] 2015 Nov; Vol. 9 (11), pp. 2537-40. Date of Electronic Publication: 2015 Apr 17.
DOI:	10.1038/ismej.2015.43
Abstrakt:	Ice-binding proteins (IBPs) have been isolated from various sea-ice organisms. Their characterisation points to a crucial role in protecting the organisms in sub-zero environments. However, their in situ abundance and diversity in natural sea-ice microbial communities is largely unknown. In this study, we analysed the expression and phylogenetic diversity of eukaryotic IBP transcripts from microbial communities of Arctic and Antarctic sea ice. IBP transcripts were found in abundances similar to those of proteins involved in core cellular processes such as photosynthesis. Eighty-nine percent of the IBP transcripts grouped with known IBP sequences from diatoms, haptophytes and crustaceans, but the majority represented novel sequences not previously characterized in cultured organisms. The observed high eukaryotic IBP expression in natural eukaryotic sea ice communities underlines the essential role of IBPs for survival of many microorganisms in communities living under the extreme conditions of polar sea ice.
Databáze:	MEDLINE
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