| Autor: |
Babich LG, Bychenok SF, Kondratiuk TP |
| Jazyk: |
ruština |
| Zdroj: |
Ukrainskii biokhimicheskii zhurnal (1978) [Ukr Biokhim Zh (1978)] 1989 Sep-Oct; Vol. 61 (5), pp. 42-6. |
| Abstrakt: |
A method is suggested for obtaining a catalytic subunit of cAMP-dependent protein kinase from the cattle myocardium. The specific activity of protein kinase is 0.5 mumol 32P per 1 mg of the enzyme. The method is based on the difference of protein kinase in the subunit and choloenzyme charges, it embraces the stages of homogenization, ultracentrifugation and biospecific elution on anion exchanger of DEAE-Sepharose-CL-6B using 10(-4) M cAMP in the stationary variant. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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