Effect of smoking on serum xanthine oxidase, malondialdehyde, ascorbic acid and α-tocopherol levels in healthy male subjects.

Autor: Shah AA; Ali Akbar Shah, M.Phil. Assistant Professor, Department of Biochemistry, Isra University Hyderabad, Sindh, Pakistan., Khand F; Fatehuddin Khand, PhD. Professor, Department of Biochemistry, Isra University Hyderabad, Sindh, Pakistan., Khand TU; Tayyab Uddin Khand, M.Phil. Assistant Professor, Department of Biochemistry, Indus Medical College, Tando Mohammad Khan, Sindh, Pakistan.
Jazyk: angličtina
Zdroj: Pakistan journal of medical sciences [Pak J Med Sci] 2015 Jan-Feb; Vol. 31 (1), pp. 146-9.
DOI: 10.12669/pjms.311.6148
Abstrakt: Objective: To examine the effect of smoking on serum xanthine oxidase, malondialdehyde, α- tocopherol and ascorbic acid levels in healthy adult male subjects.
Methods: This cross-sectional comparative study was carried out at Isra University Hyderabad from July 2012 to December 2012. One hundred and twenty apparently healthy adult male subjects (60 smokers and 60 non-smokers) included in present study, were recruited from Jaindal kot, a small village located midway between Hyderabad and Matiari. Serum samples from smokers and non-smokers were analyzed for xanthine oxidase and malondialdehyde levels by standard kit methods, while for ascorbic acid and alpha- tocopherol by spectrophotometric methods.
Results: The mean xanthine oxidase and malondialdehyde levels measured in healthy smokers were 0.30±0.05 mg/dl and 37.50±4.05 µmoles/l respectively as against 0.25±0.04 mg/dl and 19.86±2.21µmoles/l in non-smokers. Both xanthine oxidase and malondialdehyde levels were significantly (p<0.001) raised in healthy smokers than in non-smokers. Likewise, mean vitamin E and vitamin C levels were respectively 0.69±0.37 mg/dl and 0.80±0.16 mg/dl in healthy smokers compared to 1.14±0.43 mg/dl and 1.22±0.29 mg/dl in non-smokers. The concentrations of both these vitamins were significantly (p<0.001) lower in smokers than in non-smokers.
Conclusion: The results of present study demonstrate that smoking had significantly increased xanthine oxidase and malondialdehyde levels and decreased vitamins C and E (antioxidants) levels. These findings suggest that smokers have to take additional amounts of vitamins C and E in order to avoid deleterious effects of smoking on their health.
Databáze: MEDLINE
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