| Autor: |
Cherepaninets VD, Zhironkina OA, Strelkova OS, Kurchashova SIu, Kireev II |
| Jazyk: |
ruština |
| Zdroj: |
Tsitologiia [Tsitologiia] 2015; Vol. 57 (1), pp. 47-55. |
| Abstrakt: |
It is considered that sister chromatids are held together immediately after replication by special protein complex--cohesin that consists of Smc1--Smc3 core dimer and two additional subunits, Scc1 and Scc3. This process is called cohesion. We have characterized binding of cohesin complex to early- and late-replicated chromatin at different stages of the cell cycle in human cells HeLa and HT1080 using superresolution microscopy (based on Structural ilumination microscopy--SIM) and immunoelectron microscopy. It has been shown that cohesins do not play important role in cohesion of heterochromatic domains, but they provide cohesion and organization of subdomains in euchromatic regions. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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