Participation of the TRP channel in the cardiovascular effects induced by carvacrol in normotensive rat.

Autor: Dantas BP; Centro de Biotecnologia (CBiotec), Universidade Federal da Paraíba (UFPB), Cidade Universitária, Campus I, João Pessoa, PB 58051-970, Brazil., Alves QL; Departamento de Biorregulação, Instituto de Ciências da Saúde, Universidade Federal da Bahia (UFBA), Av. Reitor Miguel Calmon, S/N, Vale do Canela, Salvador, BA 40110-902, Brazil., de Assis KS; Departamento de Ciências Farmacêuticas, Universidade Federal da Paraíba (UFPB), Cidade Universitária, Campus I, João Pessoa, PB 58059-900, Brazil., Ribeiro TP; Departamento de Ciências Farmacêuticas, Universidade Federal da Paraíba (UFPB), Cidade Universitária, Campus I, João Pessoa, PB 58059-900, Brazil., de Almeida MM; Departamento de Ciências Farmacêuticas, Universidade Federal da Paraíba (UFPB), Cidade Universitária, Campus I, João Pessoa, PB 58059-900, Brazil., de Vasconcelos AP; Centro de Biotecnologia (CBiotec), Universidade Federal da Paraíba (UFPB), Cidade Universitária, Campus I, João Pessoa, PB 58051-970, Brazil., de Araújo DA; Centro de Biotecnologia (CBiotec), Universidade Federal da Paraíba (UFPB), Cidade Universitária, Campus I, João Pessoa, PB 58051-970, Brazil., de Andrade Braga V; Centro de Biotecnologia (CBiotec), Universidade Federal da Paraíba (UFPB), Cidade Universitária, Campus I, João Pessoa, PB 58051-970, Brazil., de Medeiros IA; Departamento de Ciências Farmacêuticas, Universidade Federal da Paraíba (UFPB), Cidade Universitária, Campus I, João Pessoa, PB 58059-900, Brazil., Alencar JL; Departamento de Ciências Farmacêuticas, Universidade Federal da Paraíba (UFPB), Cidade Universitária, Campus I, João Pessoa, PB 58059-900, Brazil., Silva DF; Departamento de Biorregulação, Instituto de Ciências da Saúde, Universidade Federal da Bahia (UFBA), Av. Reitor Miguel Calmon, S/N, Vale do Canela, Salvador, BA 40110-902, Brazil. Electronic address: darizy@gmail.com.
Jazyk: angličtina
Zdroj: Vascular pharmacology [Vascul Pharmacol] 2015 Apr-Jun; Vol. 67-69, pp. 48-58. Date of Electronic Publication: 2015 Apr 11.
DOI: 10.1016/j.vph.2015.02.016
Abstrakt: Carvacrol has been described as an agonist/antagonist of different transient receptor potential (TRP) channels and voltage-dependent calcium channels (Cavs). The aim of this study was to evaluate the role of Cav and TRP channels following carvacrol stimulation. Initially, in mesenteric artery rings carvacrol relaxed phenylephrine-induced contractions. Furthermore, carvacrol inhibited contraction elicited by CaCl2 in depolarizing nominally without Ca2+ medium and antagonized the contractions induced by S(-)-Bay K 8644 and inhibited Ca2+ currents indicating the inhibition of Ca2+ influx through L-type Cav. Additionally, carvacrol antagonized the contractions induced by CaCl2 in the presence of nifedipine/Cyclopiazonic acid/phenylephrine or nifedipine/Cyclopiazonic acid/KCl 60, suggesting a possible inhibition of calcium influx by store operated channels (SOCs), receptor operated channels (ROCs) and/or TRP channels. Interestingly, among the TRP channel blockers used, the effect induced by carvacrol was attenuated by Mg2+ and potentiated by La3+ and Gd3+, suggesting that TRP channels are involved in relaxation induced by carvacrol. Monoterpene also induced hypotension and bradycardia in non-anesthetized normotensive rats and negative inotropic and chronotropic effects. In conclusion, these results suggest that the hypotensive effect of carvacrol is probably due to bradycardia and a peripheral vasodilatation that involves, at least, the inhibition of the Ca2+ influx through Cav and TRP channels.
(Copyright © 2015 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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