Comparison of the CT OligoGen kit with cobas 4800 assay for detection of Chlamydia trachomatis.

Autor:	Parra-Sánchez M; Unit of Infectious Disease and Clinical Microbiology, Valme University Hospital, Seville, Spain. Electronic address: manuel.parra.exts@juntadeandalucia.es., Marcuello-López A; Operon Inmuno & Molecular Diagnostics, Cuarte de Huerva, Zaragoza, Spain., García-Rey S; Unit of Infectious Disease and Clinical Microbiology, Valme University Hospital, Seville, Spain., Zakariya-Yousef I; Unit of Infectious Disease and Clinical Microbiology, Valme University Hospital, Seville, Spain., Sivianes-Valdecantos N; Unit of Infectious Disease and Clinical Microbiology, Valme University Hospital, Seville, Spain., Sierra-Atienza C; Unit of Infectious Disease and Clinical Microbiology, Valme University Hospital, Seville, Spain., Bernal-Martínez S; Unit of Infectious Disease and Clinical Microbiology, Valme University Hospital, Seville, Spain., Pueyo-Rodrígez I; Center of Sexually Transmitted Infections of Seville, Seville, Spain., Martín-Mazuelos E; Unit of Infectious Disease and Clinical Microbiology, Valme University Hospital, Seville, Spain., Palomares-Folía JC; Unit of Infectious Disease and Clinical Microbiology, Valme University Hospital, Seville, Spain.
Jazyk:	angličtina
Zdroj:	Enfermedades infecciosas y microbiologia clinica [Enferm Infecc Microbiol Clin] 2015 Dec; Vol. 33 (10), pp. 642-5. Date of Electronic Publication: 2015 Apr 06.
DOI:	10.1016/j.eimc.2015.02.017
Abstrakt:	Introduction: A prospective study was designed to assess the performance of the new CT OligoGen kit and the cobas 4800 assay for detection of Chlamydia trachomatis. Methods: A set of samples that included urine samples (n=212), endocervical (n=167), rectal (n=53), pharyngeal (n=7) and urethral swabs (n=3). The samples were sent from a regional sexually transmitted diseases (STD) clinic in Seville, Spain, and were collected from 261 men and 181 women. Discordant results were re-analyzed and clinical data and other tests were reviewed in order to resolve them. Results: Sensitivity, specificity, positive predicative value (PPV), negative predictive value (NPV) and kappa value for C. trachomatis detection using the CT OligoGen kit were 98.5%, 100%, 100%, 95.4% and 0.97, respectively. Conclusions: This new kit had a high sensitivity, specificity, PPV and NPV for C. trachomatis, therefore the performance profile confirms the usefulness and reliable results of this new assay. (Copyright © 2015 Elsevier España, S.L.U. y Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica. All rights reserved.)
Databáze:	MEDLINE
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