Transcriptional dynamics of Phytophthora infestans during sequential stages of hemibiotrophic infection of tomato.

Autor: Zuluaga AP; Section of Plant Pathology and Plant Microbe Biology, School of Integrative Plant Science, Cornell University, Ithaca, NY, 14853, USA., Vega-Arreguín JC; Section of Plant Pathology and Plant Microbe Biology, School of Integrative Plant Science, Cornell University, Ithaca, NY, 14853, USA.; Laboratory of Agrigenomics, Universidad Nacional Autónoma de México (UNAM), ENES-León, 37684, Guanajuato, Mexico., Fei Z; Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, NY, 14853, USA.; Robert W. Holly Center for Agriculture and Health, USDA-ARS, Tower Road, Ithaca, NY, 14853, USA., Ponnala L; Institute for Biotechnology and Life Science Technologies, Cornell University, Ithaca, NY, 14853, USA., Lee SJ; Section of Plant Biology, School of Integrative Plant Science, Cornell University, Ithaca, NY, 14853, USA.; Biotechnology Institute, Nongwoo Bio Co., Ltd, Gyeonggi, South Korea., Matas AJ; Section of Plant Biology, School of Integrative Plant Science, Cornell University, Ithaca, NY, 14853, USA.; Departamento de Biología Vegetal, Campus de Teatinos, Universidad de Málaga, 29071, Málaga, Spain., Patev S; Section of Plant Pathology and Plant Microbe Biology, School of Integrative Plant Science, Cornell University, Ithaca, NY, 14853, USA., Fry WE; Section of Plant Pathology and Plant Microbe Biology, School of Integrative Plant Science, Cornell University, Ithaca, NY, 14853, USA., Rose JK; Section of Plant Biology, School of Integrative Plant Science, Cornell University, Ithaca, NY, 14853, USA.
Jazyk: angličtina
Zdroj: Molecular plant pathology [Mol Plant Pathol] 2016 Jan; Vol. 17 (1), pp. 29-41. Date of Electronic Publication: 2015 May 08.
DOI: 10.1111/mpp.12263
Abstrakt: Hemibiotrophic plant pathogens, such as the oomycete Phytophthora infestans, employ a biphasic infection strategy, initially behaving as biotrophs, where minimal symptoms are exhibited by the plant, and subsequently as necrotrophs, feeding on dead plant tissue. The regulation of this transition and the breadth of molecular mechanisms that modulate plant defences are not well understood, although effector proteins secreted by the pathogen are thought to play a key role. We examined the transcriptional dynamics of P. infestans in a compatible interaction with its host tomato (Solanum lycopersicum) at three infection stages: biotrophy; the transition from biotrophy to necrotrophy; and necrotrophy. The expression data suggest a tight temporal regulation of many pathways associated with the suppression of plant defence mechanisms and pathogenicity, including the induction of putative cytoplasmic and apoplastic effectors. Twelve of these were experimentally evaluated to determine their ability to suppress necrosis caused by the P. infestans necrosis-inducing protein PiNPP1.1 in Nicotiana benthamiana. Four effectors suppressed necrosis, suggesting that they might prolong the biotrophic phase. This study suggests that a complex regulation of effector expression modulates the outcome of the interaction.
(© 2015 BSPP AND JOHN WILEY & SONS LTD.)
Databáze: MEDLINE
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