Imaging and analyzing primary cilia in cardiac cells.

Autor:	Diguet N; Institut Pasteur, Department of Developmental and Stem Cell Biology, Paris, France; CNRS URA2578, Paris, France., Le Garrec JF; Institut Pasteur, Department of Developmental and Stem Cell Biology, Paris, France; CNRS URA2578, Paris, France., Lucchesi T; Institut Pasteur, Department of Developmental and Stem Cell Biology, Paris, France; CNRS URA2578, Paris, France; Sorbonne Universités, UPMC Université Paris06, IFD, Paris, France., Meilhac SM; Institut Pasteur, Department of Developmental and Stem Cell Biology, Paris, France; CNRS URA2578, Paris, France.
Jazyk:	angličtina
Zdroj:	Methods in cell biology [Methods Cell Biol] 2015; Vol. 127, pp. 55-73. Date of Electronic Publication: 2015 Mar 07.
DOI:	10.1016/bs.mcb.2015.01.008
Abstrakt:	The primary cilium is a small sensory organelle that is required for different aspects of embryonic development, including the formation of the heart. The structure and composition of cilia have been extensively studied, so that several markers of primary cilia have now been identified. However, the role of cilia in specific cell types remains poorly understood. We describe here a series of approaches to image primary cilia in the rodent heart or in primary cultures of cells dissociated from the heart. As the cilium is a marker of cell polarity, we also provide, for quantitative image analysis of cilium orientation, tools which are generally applicable to other types of tissues. (Copyright © 2015 Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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