RNA Polymerase II Second Largest Subunit Molecular Identification of Boletus griseipurpureus Corner From Thailand and Antibacterial Activity of Basidiocarp Extracts.

Autor:	Aung-Aud-Chariya A; Center of Excellence on Agricultural Biotechnology, Science and Technology, Bangkok, Thailand ; Rajamangala University of Technology, Srivijaya Trang Campus, Thammarat, Thailand ; School of Science, Walailak University, Nakhon Si Thammarat, Thailand., Bangrak P; School of Science, Walailak University, Nakhon Si Thammarat, Thailand., Lumyong S; Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand., Phupong W; School of Science, Walailak University, Nakhon Si Thammarat, Thailand., Aggangan NS; National Institutes of Molecular Biology and Biotechnology, University of the Philippines Los Banos, Laguna, Phillippines., Kamlangdee N; School of Science, Walailak University, Nakhon Si Thammarat, Thailand.
Jazyk:	angličtina
Zdroj:	Jundishapur journal of microbiology [Jundishapur J Microbiol] 2015 Mar 21; Vol. 8 (3), pp. e15552. Date of Electronic Publication: 2015 Mar 21 (Print Publication: 2015).
DOI:	10.5812/jjm.15552
Abstrakt:	Background: Boletus griseipurpureus Corner, an edible mushroom, is a putative ectomycorrhizal fungus. Currently, the taxonomic boundary of this mushroom is unclear and its bitter taste makes it interesting for evaluating its antibacterial properties. Objectives: The purpose of this study was to identify the genetic variation of this mushroom and also to evaluate any antibacterial activities. Materials and Methods: Basidiocarps were collected from 2 north-eastern provinces, Roi Et and Ubon Ratchathani, and from 2 southern provinces, Songkhla and Surat Thani, in Thailand. Genomic DNA was extracted and molecular structure was examined using the RNA polymerase II (RPB2) analysis. Antibacterial activities of basidiocarp extracts were conducted with Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 29523 and methicillin-resistant Staphylococcus aureus (MRSA) 189 using the agar-well diffusion method. Results: All the samples collected for this study constituted a monophyletic clade, which was closely related with the Boletus group of polypore fungi. For the antibacterial study, it was found that the crude methanol extract of basidiomes inhibited the growth of all bacteria in vitro more than the crude ethyl acetate extract. Conclusions: Basidomes collected from four locations in Thailand had low genetic variation and their extracts inhibited the growth of all tested bacteria. The health benefits of this edible species should be evaluated further.
Databáze:	MEDLINE
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