Role of stress-related hormones in plant defence during early infection of the cyst nematode Heterodera schachtii in Arabidopsis.

Autor: Kammerhofer N; Division of Plant Protection, Department of Crop Sciences, University of Natural Resources and Life Sciences, UFT Tulln, Konrad Lorenz Str. 24, 3430, Tulln, Austria., Radakovic Z; Institute of Crop Science and Resource Conservation, Department Molecular Phytomedicine, University Bonn, Karlrobert-Kreiten-Str. 13, D-53115, Bonn, Germany., Regis JM; Division of Plant Protection, Department of Crop Sciences, University of Natural Resources and Life Sciences, UFT Tulln, Konrad Lorenz Str. 24, 3430, Tulln, Austria., Dobrev P; Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojová 263, 165 02, Prague 6, Lysolaje, Czech Republic., Vankova R; Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojová 263, 165 02, Prague 6, Lysolaje, Czech Republic., Grundler FM; Institute of Crop Science and Resource Conservation, Department Molecular Phytomedicine, University Bonn, Karlrobert-Kreiten-Str. 13, D-53115, Bonn, Germany., Siddique S; Institute of Crop Science and Resource Conservation, Department Molecular Phytomedicine, University Bonn, Karlrobert-Kreiten-Str. 13, D-53115, Bonn, Germany., Hofmann J; Division of Plant Protection, Department of Crop Sciences, University of Natural Resources and Life Sciences, UFT Tulln, Konrad Lorenz Str. 24, 3430, Tulln, Austria., Wieczorek K; Division of Plant Protection, Department of Crop Sciences, University of Natural Resources and Life Sciences, UFT Tulln, Konrad Lorenz Str. 24, 3430, Tulln, Austria.
Jazyk: angličtina
Zdroj: The New phytologist [New Phytol] 2015 Aug; Vol. 207 (3), pp. 778-89. Date of Electronic Publication: 2015 Mar 30.
DOI: 10.1111/nph.13395
Abstrakt: Heterodera schachtii, a plant-parasitic cyst nematode, invades host roots and induces a specific syncytial feeding structure, from which it withdraws all required nutrients, causing severe yield losses. The system H. schachtii-Arabidopsis is an excellent research model for investigating plant defence mechanisms. Such responses are suppressed in well-established syncytia, whereas they are induced during early parasitism. However, the mechanisms by which the defence responses are modulated and the role of phytohormones are largely unknown. The aim of this study was to elucidate the role of hormone-based defence responses at the onset of nematode infection. First, concentrations of main phytohormones were quantified and the expression of several hormone-related genes was analysed using quantitative real-time (qRT)-PCR or GeneChip. Further, the effects of individual hormones were evaluated via nematode attraction and infection assays using plants with altered endogenous hormone concentrations. Our results suggest a pivotal and positive role for ethylene during nematode attraction, whereas jasmonic acid triggers early defence responses against H. schachtii. Salicylic acid seems to be a negative regulator during later syncytium and female development. We conclude that nematodes are able to impose specific changes in hormone pools, thus modulating hormone-based defence and signal transduction in strict dependence on their parasitism stage.
(© 2015 The Authors. New Phytologist © 2015 New Phytologist Trust.)
Databáze: MEDLINE
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