Repeated mechanical lengthening of intestinal segments in a novel model.

Autor: Scott A; Division of Pediatric Surgery, Department of Surgery, University of California, Los Angeles, CA, USA., Sullins VF; Division of Pediatric Surgery, Department of Surgery, University of California, Los Angeles, CA, USA., Steinberger D; Department of Bioengineering, University of California, Los Angeles, CA, USA., Rouch JD; Division of Pediatric Surgery, Department of Surgery, University of California, Los Angeles, CA, USA., Wagner JP; Division of Pediatric Surgery, Department of Surgery, University of California, Los Angeles, CA, USA., Chiang E; Division of Pediatric Surgery, Department of Surgery, University of California, Los Angeles, CA, USA., Lee SL; Division of Pediatric Surgery, Department of Surgery, University of California, Los Angeles, CA, USA., Wu BM; Department of Bioengineering, University of California, Los Angeles, CA, USA., Dunn JC; Division of Pediatric Surgery, Department of Surgery, University of California, Los Angeles, CA, USA; Department of Bioengineering, University of California, Los Angeles, CA, USA. Electronic address: jdunn@mednet.ucla.edu.
Jazyk: angličtina
Zdroj: Journal of pediatric surgery [J Pediatr Surg] 2015 Jun; Vol. 50 (6), pp. 954-7. Date of Electronic Publication: 2015 Mar 14.
DOI: 10.1016/j.jpedsurg.2015.03.020
Abstrakt: Purpose: Currently, animal models used for mechanical intestinal lengthening utilize a single lengthening procedure prior to analysis or restoration back into continuity. Here we developed a novel surgical model to examine the feasibility of repeated lengthening of intestinal segments.
Methods: A Roux-en-Y jejunojejunostomy with a blind Roux limb was created in rats. An encapsulated polycaprolactone spring was placed into a 1cm segment of the Roux limb. After 4 weeks, a second encapsulated PCL spring was inserted into a 1cm portion of the lengthened segment. After another 4 weeks, the repeatedly lengthened segments were retrieved for histological analyses.
Results: Jejunal segments of the Roux limb were successfully lengthened from 1.0 cm to 2.6 ± 0.7 cm. Four weeks after the second PCL spring placement, 1.0 cm of the previously lengthened segment increased to 2.7 ± 0.8 cm. Stronger mechanical force was required to achieve subsequent re-lengthening. Lengthened and re-lengthened segments had increased smooth muscle thickness and crypt depth when compared to normal jejunal mucosa.
Conclusion: Using the Roux-en-Y model, previously lengthened segments of intestine can be successfully re-lengthened. Intestinal segments may be subjected to multiple lengthening procedures to achieve clinically significant length for the treatment of short bowel syndrome.
(Copyright © 2015 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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