| Abstrakt: |
The effect of polypeptides isolated from cattle abomasum on regenerative processes of rat stomach upon simulating stomach mucosal damage caused by aspirin was studied. Experimental research was carried out on male Wistar rats with initial body weight of 230±20 g. The duration of the experiment was 22 days. The rats were divided into 4 equal groups (n=11). The first (control) group-consisted of the intact animals; animals from experimental groups 2-4 were intragastrically administered acetylsalicylic acid from the 1st to the 7th day for simulating stomach mucosal damage caused by aspirin (300 mg/100 g body weight). From day 8 to day 22, the animals were intragastrically adminitered the tested samples in the quantity of 2 ml per animal according tothe scheme: the 2nd group - distilled water, the 3d group - native abomasum extract; the 4 th group - thermally treated abomasum extract. Abomasum extract was obtained by extraction with 0,87% aqueous sodium chloride crushed abomasum and represented a liquid of cream color with protein mass content of 1,3 g/100 g of the product with high content of glutamic acid (15,5 g/100 g protein) and B-group vitamins. Electrophoretic analysis of the extract revealed several high molecular weight fractions in the range of 72 to 55 kDa. The bands with molecular masses 52, 43, 40, 37, 34, 26, 17 kDa were most pronounced; the intensive bands in the area 12 kDa and in the range lower than 10 kDa were revealed. The results of the conducted study show that the abomasum extracts both in the native and thermally treated form exert therapeutic action on animal with stomach mucosal damage caused by aspirin, have good antiulcer and gastroprotective activities upon stomach mucosa exposure to chemical damaging agents. The analysis of the hematological indices of the animals from the 3rd and 4th groups, which received the test samples after simulation, revealed the normalization of leukocyte, lymphocyte, granulocyte and monocyte content. This suggests the recovery of the animals after the disease. In the blood serum of these animals concentration of total bilirubin, cholesterol, triglycerides and glucose and the activity of gamma-glutamine transferase, ASAT and alkaline phosphatase decreased compared with those in animals with the model of stomach mucosal injury; while the total protein content, including the albumin fraction increased. The examination of the internal organs of the animals from the 3rd and 4th groups showed that the mucosal and submucosal membrane of the stomach were plicate, the signs of edema were absent, the hyperemia, any changes in mucosa, surface and glandular epithelium were not observed. |
