A dynamic spectrum of monocytes arising from the in situ reprogramming of CCR2+ monocytes at a site of sterile injury.

Autor:	Dal-Secco D; Immunology Research Group, Snyder Institute for Chronic Diseases; Department of Microbiology, Immunology, and Infectious Diseases; and Department of Physiology and Pharmacology, University of Calgary, Calgary, Alberta T2N 4N1, Canada., Wang J; Immunology Research Group, Snyder Institute for Chronic Diseases; Department of Microbiology, Immunology, and Infectious Diseases; and Department of Physiology and Pharmacology, University of Calgary, Calgary, Alberta T2N 4N1, Canada Immunology Research Group, Snyder Institute for Chronic Diseases; Department of Microbiology, Immunology, and Infectious Diseases; and Department of Physiology and Pharmacology, University of Calgary, Calgary, Alberta T2N 4N1, Canada., Zeng Z; Immunology Research Group, Snyder Institute for Chronic Diseases; Department of Microbiology, Immunology, and Infectious Diseases; and Department of Physiology and Pharmacology, University of Calgary, Calgary, Alberta T2N 4N1, Canada Immunology Research Group, Snyder Institute for Chronic Diseases; Department of Microbiology, Immunology, and Infectious Diseases; and Department of Physiology and Pharmacology, University of Calgary, Calgary, Alberta T2N 4N1, Canada., Kolaczkowska E; Immunology Research Group, Snyder Institute for Chronic Diseases; Department of Microbiology, Immunology, and Infectious Diseases; and Department of Physiology and Pharmacology, University of Calgary, Calgary, Alberta T2N 4N1, Canada., Wong CH; Immunology Research Group, Snyder Institute for Chronic Diseases; Department of Microbiology, Immunology, and Infectious Diseases; and Department of Physiology and Pharmacology, University of Calgary, Calgary, Alberta T2N 4N1, Canada., Petri B; Immunology Research Group, Snyder Institute for Chronic Diseases; Department of Microbiology, Immunology, and Infectious Diseases; and Department of Physiology and Pharmacology, University of Calgary, Calgary, Alberta T2N 4N1, Canada Immunology Research Group, Snyder Institute for Chronic Diseases; Department of Microbiology, Immunology, and Infectious Diseases; and Department of Physiology and Pharmacology, University of Calgary, Calgary, Alberta T2N 4N1, Canada., Ransohoff RM; Neuroinflammation Research Center, Department of Neurosciences, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195., Charo IF; Gladstone Institute of Cardiovascular Disease and Cardiovascular Research Institute, Department of Medicine, University of California, San Francisco, San Francisco, CA 94143., Jenne CN; Immunology Research Group, Snyder Institute for Chronic Diseases; Department of Microbiology, Immunology, and Infectious Diseases; and Department of Physiology and Pharmacology, University of Calgary, Calgary, Alberta T2N 4N1, Canada Immunology Research Group, Snyder Institute for Chronic Diseases; Department of Microbiology, Immunology, and Infectious Diseases; and Department of Physiology and Pharmacology, University of Calgary, Calgary, Alberta T2N 4N1, Canada pkubes@ucalgary.ca cnjenne@ucalgary.ca., Kubes P; Immunology Research Group, Snyder Institute for Chronic Diseases; Department of Microbiology, Immunology, and Infectious Diseases; and Department of Physiology and Pharmacology, University of Calgary, Calgary, Alberta T2N 4N1, Canada Immunology Research Group, Snyder Institute for Chronic Diseases; Department of Microbiology, Immunology, and Infectious Diseases; and Department of Physiology and Pharmacology, University of Calgary, Calgary, Alberta T2N 4N1, Canada pkubes@ucalgary.ca cnjenne@ucalgary.ca.
Jazyk:	angličtina
Zdroj:	The Journal of experimental medicine [J Exp Med] 2015 Apr 06; Vol. 212 (4), pp. 447-56. Date of Electronic Publication: 2015 Mar 23.
DOI:	10.1084/jem.20141539
Abstrakt:	Monocytes are recruited from the blood to sites of inflammation, where they contribute to wound healing and tissue repair. There are at least two subsets of monocytes: classical or proinflammatory (CCR2(hi)CX3CR1(low)) and nonclassical, patrolling, or alternative (CCR2(low)CX3CR1(hi)) monocytes. Using spinning-disk confocal intravital microscopy and mice with fluorescent reporters for each of these subsets, we were able to track the dynamic spectrum of monocytes that enter a site of sterile hepatic injury in vivo. We observed that the CCR2(hi)CX3CR1(low) monocytes were recruited early and persisted for at least 48 h, forming a ringlike structure around the injured area. These monocytes transitioned, in situ, from CCR2(hi)Cx3CR1(low) to CX3CR1(hi)CCR2(low) within the ringlike structure and then entered the injury site. This phenotypic conversion was essential for optimal repair. These results demonstrate a local, cytokine driven reprogramming of classic, proinflammatory monocytes into nonclassical or alternative monocytes to facilitate proper wound-healing. (© 2015 Dal-Secco et al.)
Databáze:	MEDLINE
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