Modulation of gene expression and cell cycle by botryosphaeran, a (1→3)(1→6)-β-d-glucan in human lymphocytes.

Autor: Malini M; Departmento de Biologia Geral, Centro de Ciências Biológicas - Universidade Estadual de Londrina, Londrina - PR, Brazil., Souza MF; Departmento de Biologia Geral, Centro de Ciências Biológicas - Universidade Estadual de Londrina, Londrina - PR, Brazil., Oliveira MT; Departmento de Biologia Geral, Centro de Ciências Biológicas - Universidade Estadual de Londrina, Londrina - PR, Brazil., Antunes LM; Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto - Universidade de São Paulo, Ribeirão Preto - SP, Brazil., Figueiredo SG; Departamento de Ciências Biológicas, Centro de Ciências Fisiológicas - Universidade Federal do Espírito Santo, Vitória - ES, Brazil., Barbosa AM; Departmento de Química, Centro de Ciências Exatas - Universidade Estadual de Londrina, Londrina - PR, Brazil; Biorefining Research Institute, Lakehead University, Thunder Bay, Ontario, Canada P7B 5E1., Dekker RF; Biorefining Research Institute, Lakehead University, Thunder Bay, Ontario, Canada P7B 5E1., Cólus IM; Departmento de Biologia Geral, Centro de Ciências Biológicas - Universidade Estadual de Londrina, Londrina - PR, Brazil. Electronic address: ilcecolus@gmail.com.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2015; Vol. 77, pp. 214-21. Date of Electronic Publication: 2015 Mar 18.
DOI: 10.1016/j.ijbiomac.2015.03.010
Abstrakt: There is growing interest in the anticancer and immunomodulatory potential of fungal β-d-glucans. In the present study, the modulation of gene expression via RT-qPCR and cell cycle kinetics via flow cytometry were assessed in human normal and tumor (Jurkat) lymphocytes after treatment with botryosphaeran (a fungal (1→3)(1→6)-β-d-glucan) from Botryosphaeria rhodina MAMB-05. Cell cultures were treated with botryosphaeran either alone, or in combination with doxorubicin (DXR), in a post-treatment protocol. The expression of genes involved in immunomodulatory processes, apoptosis and cell cycle control, as well as β-d-glucans cell receptors were assessed. Flow cytometry analysis identified tetraploid Jurkat cells in G1 phase when treated with botryosphaeran combined with DXR. This antiproliferative effect in G1 may be associated with down-regulation of the expression of genes involved in the G1 checkpoint. The repression of the CCR5 gene following botryosphaeran treatment, either alone or in combination with DXR, in tumor lymphocytes indicates a possible affinity of this particular (1→3)(1→6)-β-d-glucan for the receptor CCR5. Therefore, botryosphaeran action appears to be involved in the repression of genes related to the G1 phase of the cell cycle and possibly in the interaction of the botryosphaeran, either alone, or in combination with DXR, with the CCR5 receptor.
(Copyright © 2015 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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