Aging does not affect the ability of human monocyte-derived dendritic cells to phagocytose Candida albicans.

Autor: do Nascimento MP; Department of Stomatology, Bauru School of Dentistry, University of São Paulo, Al. Dr. Octávio Pinheiro Brisola, 9-75, Vila Universitária, Bauru, SP, 17012-901, Brazil., Pinke KH; Department of Stomatology, Bauru School of Dentistry, University of São Paulo, Al. Dr. Octávio Pinheiro Brisola, 9-75, Vila Universitária, Bauru, SP, 17012-901, Brazil., Penitenti M; Flow Cytometry Laboratory, Amaral Carvalho Foundation, R. Dona Silvéria, 150, Centro, Jau, SP, 17210-080, Brazil., Ikoma MR; Flow Cytometry Laboratory, Amaral Carvalho Foundation, R. Dona Silvéria, 150, Centro, Jau, SP, 17210-080, Brazil., Lara VS; Department of Stomatology, Bauru School of Dentistry, University of São Paulo, Al. Dr. Octávio Pinheiro Brisola, 9-75, Vila Universitária, Bauru, SP, 17012-901, Brazil. vanessa@fob.usp.br.
Jazyk: angličtina
Zdroj: Aging clinical and experimental research [Aging Clin Exp Res] 2015 Dec; Vol. 27 (6), pp. 785-9. Date of Electronic Publication: 2015 Mar 18.
DOI: 10.1007/s40520-015-0344-1
Abstrakt: Background: Dendritic cells (DCs) are the most potent antigen-presenting cells, playing a key role in induction of both innate and adaptive immunity. Immunosenescence refers to age-associated changes in the immune system, which may be associated with susceptibility to infections and their clinical complications. The precise effects of aging on DCs in immunity to infections are not well understood. Among the common pathogenic microorganisms, the fungus Candida albicans is an important pathogen for the development of invasive infections, especially in immunocompromised individuals, as well as during aging.
Aims: To make a comparative in vitro evaluation of the immunomodulatory function of DCs challenged with C. albicans, by phagocytosis of the fungal cells, and determine the involvement of TLR2 and TLR4 receptors. For this purpose, DCs were generated with the use of peripheral blood monocytes from healthy young and aged subjects.
Results: The phagocytosis of C. albicans is developed by DCs in TLR2- and TLR4-dependent way. This mechanism is not affected by aging.
Conclusion: Given the important role of the DCs in responses against the fungus, it is evident that if changes in phagocytosis occurred with aging, impairment in the elderly could develop. However, the evidence that phagocytosis of this fungus by DCs is not impaired with aging, brings us to the question of which are the mechanisms truly associated with the prevalence of certain diseases in the elderly.
Databáze: MEDLINE
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