BCL6 expression correlates with the t(1;19) translocation in B-lymphoblastic leukemia.

Autor: Deucher AM; From the University of California, San Francisco, and anne.deucher@ucsfmedctr.org., Qi Z; From the University of California, San Francisco, and., Yu J; From the University of California, San Francisco, and., George TI; University of New Mexico, Albuquerque., Etzell JE; From the University of California, San Francisco, and.
Jazyk: angličtina
Zdroj: American journal of clinical pathology [Am J Clin Pathol] 2015 Apr; Vol. 143 (4), pp. 547-57.
DOI: 10.1309/AJCPO4U4VYAAOTEL
Abstrakt: Objectives: Study to date suggests that BCL6 protein expression in B-cell neoplasia predominates in germinal center-derived tumors, but less is known regarding its expression in B-lymphoblastic leukemia. Therefore, we designed a comprehensive study of BCL6 expression in B-lymphoblastic leukemia.
Methods: BCL6, LMO, and HGAL protein expression in B-lymphoblastic leukemia was investigated using immunohistochemical staining of paraffin-embedded bone marrow specimens. Cryptic TCF3(E2A)-PBX1 rearrangements were investigated using interphase fluorescence in situ hybridization.
Results: Six (12%) of 52 B-lymphoblastic leukemias demonstrated BCL6 protein expression, with B-cell lymphoblastic leukemias containing a t(1;19) translocation demonstrating the strongest staining (three of three). Additional t(1;19) cases beyond the screening study showed similar results. Public microarray expression database mining showed that BCL6 messenger RNA expression levels in B-lymphoblastic leukemia correlated with the protein expression findings. Finally, other markers of B-cell development correlated with BCL6 expression in t(1;19) B-lymphoblastic leukemia cases, with LMO2 and HGAL proteins expressed in six (67%) of nine and eight (89%) of nine cases, respectively.
Conclusions: BCL6 expression is present in a subset of B-lymphoblastic leukemias, especially in cases containing the 1;19 translocation. Investigation for TCF3(E2A)-PBX1 rearrangements may be useful in BCL6-positive B-lymphoblastic leukemia.
(Copyright© by the American Society for Clinical Pathology.)
Databáze: MEDLINE