Efficacy of low concentrations of sodium hypochlorite and low-powered Er,Cr:YSGG laser activated irrigation against an Enterococcus faecalis biofilm.

Autor: Christo JE; Discipline of Endodontics, The University of Adelaide, Adelaide, SA, Australia., Zilm PS; Oral Microbiology Laboratory, School of Dentistry, The University of Adelaide, Adelaide, SA, Australia., Sullivan T; Discipline of Public Health, The University of Adelaide, Adelaide, SA, Australia., Cathro PR; Discipline of Endodontics, The University of Adelaide, Adelaide, SA, Australia.
Jazyk: angličtina
Zdroj: International endodontic journal [Int Endod J] 2016 Mar; Vol. 49 (3), pp. 279-86. Date of Electronic Publication: 2015 Apr 01.
DOI: 10.1111/iej.12447
Abstrakt: Aim: To establish the antibacterial efficacy of low concentrations of sodium hypochlorite with and without Er,Cr:YSGG laser activation on Enterococcus faecalis biofilms in extracted teeth.
Methodology: The root canals of 96 decoronated single-rooted extracted human teeth were prepared to a size 40, 0.06 taper 1 mm beyond the apex. They were mounted within a flow cell, which was sterilized before pumping a nutrient media through the root canals. The flow cell was inoculated with E. faecalis (ATCC 700802) and cultivated for 4 weeks. The root-ends were sealed, and the roots were then subjected to one of six treatment groups: group 1: syringe irrigation (SI) with saline (control) using a 27 -gauge Monoject needle 1 mm from the apex for 2 min; group 2: as for group 1 but with 1% NaOCl; group 3: as for group 1 but with 4% NaOCl; group 4: 0.5% NaOCl irrigation for 15 s followed by laser-activated irrigation (LAI) with four 15-s cycles replenishing the irrigant between cycles; group 5: as for group 4 but with 1% NaOCl as the irrigant; group 6: as for group 4 but with 4% NaOCl as the irrigant. Following treatment, teeth were crushed and viable bacteria were quantitated by serial dilution and plating. The colony-forming unit values were compared between groups using one-way anova and Tukey-adjusted post hoc tests. A two-tailed P value of <0.05 was considered statistically significant.
Results: The mean number of cells recovered from the 1% NaOCl SI group was significantly higher than that from the 4% NaOCl LAI group (P = 0.02).
Conclusion: Within the limitations of this laboratory study, low-powered (0.5 W) Er,Cr:YSGG laser activation did not improve the antibacterial effect of low concentrations of sodium hypochlorite.
(© 2015 International Endodontic Journal. Published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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