A semi-automated mass spectrometric immunoassay coupled to selected reaction monitoring (MSIA-SRM) reveals novel relationships between circulating PCSK9 and metabolic phenotypes in patient cohorts.

Autor: Gauthier MS; Institut de Recherches Cliniques de Montréal (affiliated to the Université de Montréal), 110 Avenue des Pins Ouest, Montréal, QC H2W 1R7, Canada., Pérusse JR; Institut de Recherches Cliniques de Montréal (affiliated to the Université de Montréal), 110 Avenue des Pins Ouest, Montréal, QC H2W 1R7, Canada., Awan Z; King Abdulaziz University, Jeddah, Saudi Arabia; McGill University, Montréal, QC H3A 1A1, Canada., Bouchard A; Institut de Recherches Cliniques de Montréal (affiliated to the Université de Montréal), 110 Avenue des Pins Ouest, Montréal, QC H2W 1R7, Canada., Tessier S; Institut de Recherches Cliniques de Montréal (affiliated to the Université de Montréal), 110 Avenue des Pins Ouest, Montréal, QC H2W 1R7, Canada., Champagne J; Institut de Recherches Cliniques de Montréal (affiliated to the Université de Montréal), 110 Avenue des Pins Ouest, Montréal, QC H2W 1R7, Canada., Krastins B; Thermo Fisher Scientific, Biomarker Research Initiatives in Mass Spectrometry Center, 790 Memorial Drive, Cambridge, MA 02139, USA., Byram G; Thermo Fisher Scientific, Biomarker Research Initiatives in Mass Spectrometry Center, 790 Memorial Drive, Cambridge, MA 02139, USA., Chabot K; Institut de Recherches Cliniques de Montréal (affiliated to the Université de Montréal), 110 Avenue des Pins Ouest, Montréal, QC H2W 1R7, Canada., Garneau P; General Surgery Department, Hôpital du Sacré-Coeur de Montréal, Montréal, QC H4J 1C5, Canada., Rabasa-Lhoret R; Institut de Recherches Cliniques de Montréal (affiliated to the Université de Montréal), 110 Avenue des Pins Ouest, Montréal, QC H2W 1R7, Canada., Faubert D; Institut de Recherches Cliniques de Montréal (affiliated to the Université de Montréal), 110 Avenue des Pins Ouest, Montréal, QC H2W 1R7, Canada., Lopez MF; Thermo Fisher Scientific, Biomarker Research Initiatives in Mass Spectrometry Center, 790 Memorial Drive, Cambridge, MA 02139, USA., Seidah NG; Institut de Recherches Cliniques de Montréal (affiliated to the Université de Montréal), 110 Avenue des Pins Ouest, Montréal, QC H2W 1R7, Canada., Coulombe B; Institut de Recherches Cliniques de Montréal (affiliated to the Université de Montréal), 110 Avenue des Pins Ouest, Montréal, QC H2W 1R7, Canada; Department of Biochemistry, Université de Montréal, Montréal, QC H3T 1J4, Canada. Electronic address: benoit.coulombe@ircm.qc.ca.
Jazyk: angličtina
Zdroj: Methods (San Diego, Calif.) [Methods] 2015 Jun 15; Vol. 81, pp. 66-73. Date of Electronic Publication: 2015 Mar 11.
DOI: 10.1016/j.ymeth.2015.03.003
Abstrakt: Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a key regulator of circulating low density lipoprotein cholesterol (LDL-C) levels. Besides its full-length mature form, multiple variants of PCSK9 have been reported such as forms that are truncated, mutated and/or with posttranslational modifications (PTMs). Previous studies have demonstrated that most of these variants affect PCSK9's function and thereby LDL-C levels. Commercial ELISA kits are available for quantification of PCSK9, but do not allow discrimination between the various forms and PTMs of the protein. To address this issue and given the complexity and wide dynamic range of the plasma proteome, we have developed a mass spectrometric immunoassay coupled to selected reaction monitoring (MSIA-SRM) for the multiplexed quantification of several forms of circulating PCSK9 in human plasma. Our MSIA-SRM assay quantifies peptides spanning the various protein domains and the S688 phosphorylation site. The assay was applied in two distinct cohorts of obese patients and healthy pregnant women stratified by their circulating LDL-C levels. Seven PCSK9 peptides were monitored in plasma samples: one in the prodomain prior to the autocleavage site at Q152, one in the catalytic domain prior to the furin cleavage site at R218, two in the catalytic domain following R218, one in the cysteine and histidine rich domain (CHRD) and the C-terminal peptide phosphorylated at S688 and unmodified. The latter was not detectable in sufficient amounts to be quantified in human plasma. All peptides were measured with high reproducibility and with LLOQ and LOD below the clinical range. The abundance of 5 of the 6 detectable PCSK9 peptides was higher in obese patients stratified with high circulating LDL-C levels as compared to those with low LDL-C (p < 0.05). The same 5 peptides showed good and statistically significant correlations with LDL-C levels (0.55 < r < 0.65; 0.0002 ⩽ p ⩽ 0.002), but not the S688 phosphorylated peptide. However, this phosphopeptide was significantly correlated with insulin resistance (r = 0.48; p = 0.04). In the pregnant women cohort, none of the peptides were associated to LDL-C levels. However, the 6 detectable PCSK9 peptides, but not PCSK9 measured by ELISA, were significantly correlated with serum triglyceride levels in this cohort. Our results also suggest that PCSK9 circulates with S688 phosphorylated at high stoichiometry. In summary, we have developed and applied a robust and sensitive MSIA-SRM assay for the absolute quantification of all PCSK9 domains and a PTM in human plasma. This assay revealed novel relationships between PCSK9 and metabolic phenotypes, as compared to classical ELISA assays.
(Copyright © 2015 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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