Enriched inorganic compounds in diesel exhaust particles induce mitogen-activated protein kinase activation, cytoskeleton instability, and cytotoxicity in human bronchial epithelial cells.

Autor: Seriani R; Laboratory of Experimental Air Pollution (LIM05), Department of Pathology, School of Medicine, University of São Paulo, São Paulo, Brazil., Junqueira MS; Central Biotery Laboratory, School of Medicine, University of São Paulo, São Paulo, Brazil., Carvalho-Sousa CE; Laboratory of Chronopharmacology, Department of Physiology, Institute of Bioscience, University of São Paulo, São Paulo, São Paulo, Brazil., Arruda AC; Experimental Therapeutics Laboratory, Department of Medicine, School of Medicine, University of São Paulo, Brazil., Martinez D; Laboratory of Microrheology and Molecular Physiology, Institute of Physics, University of São Paulo, São Paulo, Brazil., Alencar AM; Laboratory of Microrheology and Molecular Physiology, Institute of Physics, University of São Paulo, São Paulo, Brazil., Garippo AL; PREMIUM Confocal Microscopy Core Facility, University of São Paulo, Brazil., Brito JM; Laboratory of Experimental Air Pollution (LIM05), Department of Pathology, School of Medicine, University of São Paulo, São Paulo, Brazil., Martins MA; Experimental Therapeutics Laboratory, Department of Medicine, School of Medicine, University of São Paulo, Brazil., Saldiva PH; Laboratory of Experimental Air Pollution (LIM05), Department of Pathology, School of Medicine, University of São Paulo, São Paulo, Brazil., Negri EM; Laboratory of Experimental Air Pollution (LIM05), Department of Pathology, School of Medicine, University of São Paulo, São Paulo, Brazil., Mauad T; Laboratory of Experimental Air Pollution (LIM05), Department of Pathology, School of Medicine, University of São Paulo, São Paulo, Brazil., Macchione M; Laboratory of Experimental Air Pollution (LIM05), Department of Pathology, School of Medicine, University of São Paulo, São Paulo, Brazil. Electronic address: mmacchione@lim05.fm.usp.br.
Jazyk: angličtina
Zdroj: Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie [Exp Toxicol Pathol] 2015 Apr; Vol. 67 (4), pp. 323-9. Date of Electronic Publication: 2015 Mar 11.
DOI: 10.1016/j.etp.2015.02.004
Abstrakt: This study assessed the effects of the diesel exhaust particles on ERK and JNK MAPKs activation, cell rheology (viscoelasticity), and cytotoxicity in bronchial epithelial airway cells (BEAS-2B). Crude DEP and DEP after extraction with hexane (DEP/HEX) were utilized. The partial reduction of some DEP/HEX organics increased the biodisponibility of many metallic elements. JNK and ERK were activated simultaneously by crude DEP with no alterations in viscoelasticity of the cells. Mitochondrial activity, however, revealed a decrease through the MTT assay. DEP/HEX treatment increased viscoelasticity and cytotoxicity (membrane damage), and also activated JNK. Our data suggest that the greater bioavailability of metals could be involved in JNK activation and, consequently, in the reduction of fiber coherence and increase in the viscoelasticity and cytotoxicity of BEAS cells. The adverse findings detected after exposure to crude DEP and to DEP/HEX reflect the toxic potential of diesel compounds. Considering the fact that the cells of the respiratory epithelium are the first line of defense between the body and the environment, our data contribute to a better understanding of the pathways leading to respiratory cell injury and provide evidence for the onset of or worsening of respiratory diseases caused by inorganic compounds present in DEP.
(Copyright © 2015 Elsevier GmbH. All rights reserved.)
Databáze: MEDLINE
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