Bioprospection of cytotoxic compounds in fungal strains recovered from sediments of the Brazilian coast.

Autor: Rodrigues BS; Departamento de Química Orgânica e Inorgânica, Universidade Federal do Ceará, Campus do Pici, P.O. Box 6044, 60.455-970, Fortaleza-CE, Brazil (phone: +55-85-33669144)., Sahm BD, Jimenez PC, Pinto FC, Mafezoli J, Mattos MC, Rodrigues-Filho E, Pfenning LH, Abreu LM, Costa-Lotufo LV, Oliveira MC
Jazyk: angličtina
Zdroj: Chemistry & biodiversity [Chem Biodivers] 2015 Mar; Vol. 12 (3), pp. 432-42.
DOI: 10.1002/cbdv.201400193
Abstrakt: The cytotoxic activities of extracts (50 μg/ml) from 48 fungal strains, recovered from sediments of Pecém's offshore port terminal (Northeast coast of Brazil), against HCT-116 colon cancer cell lines were investigated. The most promising extract was obtained from strain BRF082, identified as Dichotomomyces cejpii by phylogenetic analyses of partial RPB2 gene sequence. Thus, it was selected for bioassay-guided isolation of the cytotoxic compounds. Large-scale fermentation of BRF082 in potato dextrose broth, followed by chromatographic purification of the bioactive fractions from the liquid medium, yielded gliotoxin (4) and its derivatives acetylgliotoxin G (3), bis(dethio)bis(methylsulfanyl)gliotoxin (1), acetylgliotoxin (5), 6-acetylbis(dethio)bis(methylsulfanyl)gliotoxin (2), besides the quinazolinone alkaloid fiscalin B. All isolated compounds were tested for their cytotoxicities against the tumor cell lines HCT-116, revealing 4 and 3 as the most cytotoxic ones (IC50 0.41 and 1.06 μg/ml, resp.).
(Copyright © 2015 Verlag Helvetica Chimica Acta AG, Zürich.)
Databáze: MEDLINE
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