In vitro evaluation of anti-herpes simplex-1 activity of three standardized medicinal plants from Lamiaceae.

Autor:	Ansari M; Pharmaceutics Research Center, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran., Sharififar F; Herbal and Traditional Medicines Research Center, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran., Arabzadeh AM; Department of Microbiology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran., Mehni F; Herbal and Traditional Medicines Research Center, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran., Mirtadzadini M; International Centers for Science and Technology and Environmental Sciences (HITEC), Kerman, Iran., Iranmanesh Z; Department of Microbiology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran., Nikpour N; Department of Microbiology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
Jazyk:	angličtina
Zdroj:	Ancient science of life [Anc Sci Life] 2014 Jul-Sep; Vol. 34 (1), pp. 33-8.
DOI:	10.4103/0257-7941.150777
Abstrakt:	Background: Rosmarinic acid (RA) is a phenolic acid with antioxidant and anti-viral effects. We have studied anti-herpes simplex virus type 1 (HSV-1) effect of three medicinal plants from Lamiaceae family which have been standardized on the basis of RA content. Materials and Methods: Methanolic extract of Teucrium polium, Ziziphora clinopoides, and Salvia rhytidea was prepared by maceration method and RA content of the plants was determined using a spectrophotometric method. Maximum nontoxic concentration (MNTC) of the extracts was determined using neutral red method. Serial dilutions of extracts up to MNTC were examined on Vero cells for anti-HSV-1 effect by plaque assay in comparison to acyclovir as a positive control. Results: Among the tested extracts, T. polium contained the highest percentage of RA (1.8%w/w) and exhibited the least toxicity (MNTC = 1000 μg/ml). The greatest anti-HSV-1 was shown by T. polium and Z. clinopoides extracts which exhibited both time and concentration-dependent plaque inhibition. Conclusion: Considering the low toxicity and significant anti-viral effect of T. polium extract, this plant would prove valuable as an active anti-viral drug.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu