| Autor: |
Hoff SD; Department of General Surgery, University of Texas M. D. Anderson Cancer Center, Houston 77030., Matsushita Y, Ota DM, Cleary KR, Yamori T, Hakomori S, Irimura T |
| Jazyk: |
angličtina |
| Zdroj: |
Cancer research [Cancer Res] 1989 Dec 15; Vol. 49 (24 Pt 1), pp. 6883-8. |
| Abstrakt: |
We collected a total of 78 tissue specimens, including primary colorectal carcinoma, normal colonic mucosa, and liver metastases of colon carcinoma, to examine whether the extracts of these tissues inhibited the binding of a monoclonal antibody FH6, specific for sialyl-dimeric LeX antigen. The results of inhibition assays demonstrated that: (a) contents of FH6-reactive molecules were greater in carcinoma tissues than in normal colonic mucosa; (b) metastatic foci in livers contained more FH6-reactive molecules than primary tumors; (c) primary tumors from Dukes' stage B1 patients contained less FH6-reactive molecules than primary tumors from Dukes' stage D patients. The inhibitory activity of these tumor tissue extracts against the binding of a monoclonal antibody FH6 to cultured colon carcinoma cells was eliminated by prior treatment of the extracts with sialidase, confirming that the FH6-reactive materials were sialyl-dimeric LeX antigen. Electrophoretic separation of tumor tissue extracts on 3% polyacrylamide gels followed by direct staining with monoclonal antibody FH6 revealed that very high molecular weight glycoproteins, presumably mucins, contained sialyl-dimeric LeX antigen. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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