Phylogenetic Clades 6 and 8 of Enterohemorrhagic Escherichia coli O157:H7 With Particular stx Subtypes are More Frequently Found in Isolates From Hemolytic Uremic Syndrome Patients Than From Asymptomatic Carriers.

Autor: Iyoda S; Department of Bacteriology I , National Institute of Infectious Diseases , Tokyo , Japan., Manning SD; Department of Microbiology and Molecular Genetics , Michigan State University , East Lansing., Seto K; Division of Bacteriology , Osaka Prefectural Institute of Public Health , Japan., Kimata K; Department of Bacteriology , Toyama Institute of Health , Japan., Isobe J; Department of Bacteriology , Toyama Institute of Health , Japan., Etoh Y; Fukuoka Institute of Health and Environmental Sciences , Japan., Ichihara S; Fukuoka Institute of Health and Environmental Sciences , Japan., Migita Y; Nagasaki Prefectural Institute for Environmental Research and Public Health , Japan., Ogata K; Oita Prefectural Institute of Health and Environment , Japan., Honda M; Fukuoka City Institute for Hygiene and the Environment , Japan., Kubota T; Microorganism Section , Kitakyusyu City Institute of Environmental Sciences , Fukuoka , Japan., Kawano K; Miyazaki Prefectural Institute for Public Health and Environment , Japan., Matsumoto K; Kumamoto Prefectural Meat Inspection Office , Japan., Kudaka J; Okinawa Prefectural Institute of Health and Environment , Japan., Asai N; Kyoto Prefectural Institute of Public Health and Environment , Japan., Yabata J; Yamaguchi Prefectural Institute of Public Health and Environment , Japan., Tominaga K; Yamaguchi Prefectural Institute of Public Health and Environment , Japan., Terajima J; Department of Bacteriology I , National Institute of Infectious Diseases , Tokyo , Japan ; Division of Microbiology , National Institute of Health Sciences , Tokyo , Japan., Morita-Ishihara T; Department of Bacteriology I , National Institute of Infectious Diseases , Tokyo , Japan., Izumiya H; Department of Bacteriology I , National Institute of Infectious Diseases , Tokyo , Japan., Ogura Y; Department of Genomics and Bioenvironmental Science, Frontier Science Research Center , University of Miyazaki , Japan., Saitoh T; Infectious Disease Surveillance Center , National Institute of infectious Diseases , Tokyo , Japan., Iguchi A; Interdisciplinary Research Organization , University of Miyazaki , Japan., Kobayashi H; National Agriculture and Food Research Organization , National Institute of Animal Health , Ibaraki , Japan., Hara-Kudo Y; Division of Microbiology , National Institute of Health Sciences , Tokyo , Japan., Ohnishi M; Department of Bacteriology I , National Institute of Infectious Diseases , Tokyo , Japan., Arai R, Kawase M, Asano Y, Asoshima N, Chiba K, Furukawa I, Kuroki T, Hamada M, Harada S, Hatakeyama T, Hirochi T, Sakamoto Y, Hiroi M, Takashi K, Horikawa K, Iwabuchi K, Kameyama M, Kasahara H, Kawanishi S, Kikuchi K, Ueno H, Kitahashi T, Kojima Y, Konishi N, Obata H, Kai A, Kono T, Kurazono T, Matsumoto M, Matsumoto Y, Nagai Y, Naitoh H, Nakajima H, Nakamura H, Nakane K, Nishi K, Saitoh E, Satoh H, Takamura M, Shiraki Y, Tanabe J, Tanaka K, Tokoi Y, Yatsuyanagi J
Jazyk: angličtina
Zdroj: Open forum infectious diseases [Open Forum Infect Dis] 2014 Aug 12; Vol. 1 (2), pp. ofu061. Date of Electronic Publication: 2014 Aug 12 (Print Publication: 2014).
DOI: 10.1093/ofid/ofu061
Abstrakt: Background: Enterohemorrhagic Escherichia coli (EHEC) O157:H7 infection causes severe diseases such as bloody diarrhea and hemolytic uremic syndrome (HUS). Although EHEC O157:H7 strains have exhibited high genetic variability, their abilities to cause human diseases have not been fully examined.
Methods: Clade typing and stx subtyping of EHEC O157:H7 strains, which were isolated in Japan during 1999-2011 from 269 HUS patients and 387 asymptomatic carriers (ACs) and showed distinct pulsed-field gel electrophoresis patterns, were performed to determine relationships between specific lineages and clinical presentation.
Results: Clades 6 and 8 strains were more frequently found among the isolates from HUS cases than those from ACs (P = .00062 for clade 6, P < .0001 for clade 8). All clade 6 strains isolated from HUS patients harbored stx2a and/or stx2c, whereas all clade 8 strains harbored either stx2a or stx2a/stx2c. However, clade 7 strains were predominantly found among the AC isolates but less frequently found among the HUS isolates, suggesting a significant association between clade 7 and AC (P < .0001). Logistic regression analysis revealed that 0-9 year old age is a significant predictor of the association between clade 8 and HUS. We also found an intact norV gene, which encodes for a nitric oxide reductase that inhibits Shiga toxin activity under anaerobic condition, in all clades 1-3 isolates but not in clades 4-8 isolates.
Conclusions: Early detection of EHEC O157:H7 strains that belonged to clades 6/8 and harbored specific stx subtypes may be important for defining the risk of disease progression in EHEC-infected 0- to 9-year-old children.
Databáze: MEDLINE
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