Enzymatic biodiesel synthesis from yeast oil using immobilized recombinant Rhizopus oryzae lipase.

Autor: Duarte SH; Laboratory of Bioprocess Engineering, Faculty of Food Engineering - UNICAMP, Campinas, Brazil. Electronic address: susanduarte@hotmail.com., Hernández GL; Department of Chemical Engineering, School of Engineering - Universitat Autònoma de Barcelona, 08193 Bellaterra (Barcelona), Spain., Canet A; Department of Chemical Engineering, School of Engineering - Universitat Autònoma de Barcelona, 08193 Bellaterra (Barcelona), Spain., Benaiges MD; Department of Chemical Engineering, School of Engineering - Universitat Autònoma de Barcelona, 08193 Bellaterra (Barcelona), Spain., Maugeri F; Laboratory of Bioprocess Engineering, Faculty of Food Engineering - UNICAMP, Campinas, Brazil., Valero F; Department of Chemical Engineering, School of Engineering - Universitat Autònoma de Barcelona, 08193 Bellaterra (Barcelona), Spain.
Jazyk: angličtina
Zdroj: Bioresource technology [Bioresour Technol] 2015 May; Vol. 183, pp. 175-80. Date of Electronic Publication: 2015 Feb 16.
DOI: 10.1016/j.biortech.2015.01.133
Abstrakt: The recombinant Rhizopus oryzae lipase (1-3 positional selective), immobilized on Relizyme OD403, has been applied to the production of biodiesel using single cell oil from Candida sp. LEB-M3 growing on glycerol from biodiesel process. The composition of microbial oil is quite similar in terms of saponifiable lipids than olive oil, although with a higher amount of saturated fatty acids. The reaction was carried out in a solvent system, and n-hexane showed the best performance in terms of yield and easy recovery. The strategy selected for acyl acceptor addition was a stepwise methanol addition using crude and neutralized single cell oil, olive oil and oleic acid as substrates. A FAMEs yield of 40.6% was obtained with microbial oils lower than olive oil 54.3%. Finally in terms of stability, only a lost about 30% after 6 reutilizations were achieved.
(Copyright © 2015 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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