Effects of blocking αvβ3 integrin by a recombinant RGD disintegrin on remodeling of wound healing after induction of incisional hernia in rats.
| Autor: | Oliveira CR; Department of Medicine, Federal University of Sao Carlos, Sao Carlos, SP, Brazil., Marqueti Rde C; Department of Physiological Sciences, Federal University of Brasilia, Brasilia, DF, Brazil., Cominetti MR; Department of Gerontology, UFSCar, Sao Carlos, SP, Brazil., Douat ES; Laboratory of Biochemistry and Molecular Biology, Department of Physiological Sciences, UFSCar, Sao Carlos, SP, Brazil., Ribeiro JU; Department of Chemistry, UFSCar, Sao Carlos, SP, Brazil., Pontes CL; Laboratory of Cell and Molecular Biology of Cancer, Department of Physiological Sciences, College of Medicine of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, SP, Brazil., Borghi-Silva A; Cardiopulmonary Physiotherapy Laboratory, UFSCar, Sao Carlos, SP, Brasil., Selistre-de-Araujo HS; Laboratory of Biochemistry and Molecular Biology, Department of Physiological Sciences, UFSCar, Sao Carlos, SP, Brazil. |
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| Jazyk: | angličtina |
| Zdroj: | Acta cirurgica brasileira [Acta Cir Bras] 2015 Feb; Vol. 30 (2), pp. 134-42. |
| DOI: | 10.1590/S0102-86502015002000008 |
| Abstrakt: | Purpose: To investigate the changes induced by DisBa-01 on repair of wound healing after induced incisional hernia (IH) in rats. Methods: Thirty two male albino rats were submitted to IH and divided into four experimental groups: G1, placebo control; G2, DisBa-01-treated; G3, anti-αvβ3 antibodies-treated and G4, anti-α2 antibodies-treated. Histological, biochemical and extracellular matrix remodeling analysis of abdominal wall were evaluated. Results: After 14 days, 100% of the G2 did not present hernia, and the hernia ring was closed by a thin membrane. In contrast, all groups maintained incisional hernia. DisBa-01 also increased the number macrophages and fibroblasts and induced the formation of new vessels. Additionally, MMP-2 was strongly activated only in G2 (p<0.05). Anti- αvβ3-integrin antibodies produced similar results than DisBa-01 but not anti-α2 integrin blocking antibodies. Conclusion: DisBa-01 has an important role in the control of wound healing and the blocking of this integrin may be an interesting therapeutically strategy in incisional hernia. |
| Databáze: | MEDLINE |
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