[Bioremediation of oil-polluted soils: using the [13C]/[12C] ratio to characterize microbial products of oil hydrocarbon biodegradation].

Autor: Ziakun AM, Brodskiĭ ES, Baskunov BP, Zakharchenko VN, Peshenko VP, Filonov AE, Vetrova AA, Ivanova AA, Boronin AM
Jazyk: ruština
Zdroj: Prikladnaia biokhimiia i mikrobiologiia [Prikl Biokhim Mikrobiol] 2014 Sep-Oct; Vol. 50 (5), pp. 497-507.
DOI: 10.7868/s055510991404031x
Abstrakt: We compared data on the extent of bioremediation in soils polluted with oil. The data were obtained using conventional methods of hydrocarbon determination: extraction gas chromatography-mass spectrometry, extraction IR spectroscopy, and extraction gravimetry. Due to differences in the relative abundances of the stable carbon isotopes (13C/12C) in oil and in soil organic matter, these ratios could be used as natural isotopic labels of either substance. Extraction gravimetry in combination with characteristics of the carbon isotope composition of organic products in the soil before and after bioremediation was shown to be the most informative approach to an evaluation of soil bioremediation. At present, it is the only method enabling quantification of the total petroleum hydrocarbons in oil-polluted soil, as well as of the amounts of hydrocarbons remaining after bioremediation and those microbially transformed into organic products and biomass.
Databáze: MEDLINE