| Autor: |
Maheshwari Y; Division of Plant Pathology, Advanced Centre for Plant Virology, Indian Agricultural Research Institute, New Delhi, 110012, India., Vijayanandraj S, Jain RK, Mandal B |
| Jazyk: |
angličtina |
| Zdroj: |
Archives of virology [Arch Virol] 2015 May; Vol. 160 (5), pp. 1297-301. Date of Electronic Publication: 2015 Feb 20. |
| DOI: |
10.1007/s00705-015-2345-y |
| Abstrakt: |
Few studies have been done on engineered antibodies for diagnosis of tospovirus infections. The present study was undertaken to develop a single-chain variable fragment (scFv) for specific diagnosis of infection by groundnut bud necrosis virus (GBNV), the most prevalent serogroup IV tospovirus in India. Heavy chain (372 nucleotide [nt]) and light chain (363 nt) variable region clones obtained from a hybridoma were used to make an scFv construct that expressed a ~29-kDa protein in E. coli. The scFv specifically detected GBNV in field samples of cowpea, groundnut, mung bean, and tomato, and it did not recognize watermelon bud necrosis virus, a close relative of GBNV belonging to tospovirus serogroup IV. This study for the first time demonstrated the application of a functional scFv against a serogroup-IV tospovirus. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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