Coherent anti-Stokes Raman scattering and two photon excited fluorescence for neurosurgery.

Autor: Romeike BF; Institute of Pathology, Jena University Hospital, Friedrich-Schiller-University, Erlanger Allee 101, D-07740 Jena, Germany. Electronic address: bernd.romeike@med.uni-jena.de., Meyer T; Institute of Photonic Technology (IPHT) Jena e.V., Albert-Einstein-Straße 9, D-07745 Jena, Germany., Reichart R; Clinic for Neurosurgery, Jena University Hospital, Friedrich-Schiller-University, Erlanger Allee 101, D-07740 Jena, Germany., Kalff R; Clinic for Neurosurgery, Jena University Hospital, Friedrich-Schiller-University, Erlanger Allee 101, D-07740 Jena, Germany., Petersen I; Institute of Pathology, Jena University Hospital, Friedrich-Schiller-University, Erlanger Allee 101, D-07740 Jena, Germany., Dietzek B; Institute of Photonic Technology (IPHT) Jena e.V., Albert-Einstein-Straße 9, D-07745 Jena, Germany; Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller-University Jena, Helmholtzweg 4, D-07743 Jena, Germany., Popp J; Institute of Photonic Technology (IPHT) Jena e.V., Albert-Einstein-Straße 9, D-07745 Jena, Germany; Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller-University Jena, Helmholtzweg 4, D-07743 Jena, Germany.
Jazyk: angličtina
Zdroj: Clinical neurology and neurosurgery [Clin Neurol Neurosurg] 2015 Apr; Vol. 131, pp. 42-6. Date of Electronic Publication: 2015 Jan 31.
DOI: 10.1016/j.clineuro.2015.01.022
Abstrakt: Objective: There is no established method for in vivo imaging during biopsy and surgery of the brain, which is capable to generate competitive images in terms of resolution and contrast comparable with histopathological staining.
Methods: Coherent anti-Stokes Raman scattering (CARS) and two photon excited fluorescence (TPEF) microscopy are non-invasive all optical imaging techniques that are capable of high resolution, label-free, real-time, nondestructive examination of living cells and tissues. They provide image contrast based on the molecular composition of the specimen which allows the study of large tissue areas of frozen tissue sections ex vivo.
Results: Here, preliminary data on 55 lesions of the central nervous system are presented. The generated images very nicely demonstrate cytological and architectural features required for pathological tumor typing and grading. Furthermore, information on the molecular content of a probe is provided. The tool will be implemented into a biopsy needle or endoscope in the near future for in vivo studies.
Conclusion: With this promising multimodal imaging approach the neurosurgeon might directly see blood vessels to minimize the risk for biopsy associated hemorrhages. The attending neuropathologist might directly identify the tumor and guide the selection of representative specimens for further studies. Thus, collection of non-representative material could be avoided and the risk to injure eloquent brain tissue minimized.
(Copyright © 2015 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: