[MicroRNA-10a expression in FAB different subtype of acute myeloid leukemia and its relationship with drug resistance].

Autor:	Zhi YJ; Department of Hematology, The Third Hospital Affiliated to Soochow University, Changzhou 213003, Jiangsu Province, China., Zhi F; Research Center of Modern Medicine, The Third Hospital Affiliated to Soochow University, Changzhou 213003, Jiangsu Province, China., Wang R; Research Center of Modern Medicine, The Third Hospital Affiliated to Soochow University, Changzhou 213003, Jiangsu Province, China., Xue L; Research Center of Modern Medicine, The Third Hospital Affiliated to Soochow University, Changzhou 213003, Jiangsu Province, China., Gu WY; Department of Hematology, The Third Hospital Affiliated to Soochow University, Changzhou 213003, Jiangsu Province, China., Wang B; Department of Hematology, The Third Hospital Affiliated to Soochow University, Changzhou 213003, Jiangsu Province, China., Dong WM; Department of Hematology, The Third Hospital Affiliated to Soochow University, Changzhou 213003, Jiangsu Province, China., Li HQ; Department of Hematology, The Third Hospital Affiliated to Soochow University, Changzhou 213003, Jiangsu Province, China., Ling Y; Department of Hematology, The Third Hospital Affiliated to Soochow University, Changzhou 213003, Jiangsu Province, China., Qiu GQ; Department of Hematology, The Third Hospital Affiliated to Soochow University, Changzhou 213003, Jiangsu Province, China., Wang ZL; Department of Hematology, The Third Hospital Affiliated to Soochow University, Changzhou 213003, Jiangsu Province, China., Cao XS; Department of Hematology, The Third Hospital Affiliated to Soochow University, Changzhou 213003, Jiangsu Province, China., Liu Y; Department of Hematology, The Third Hospital Affiliated to Soochow University, Changzhou 213003, Jiangsu Province, China. E-mail:15051973544@163.com.
Jazyk:	čínština
Zdroj:	Zhongguo shi yan xue ye xue za zhi [Zhongguo Shi Yan Xue Ye Xue Za Zhi] 2015 Feb; Vol. 23 (1), pp. 29-33.
DOI:	10.7534/j.issn.1009-2137.2015.01.006
Abstrakt:	Objective: This study was to investigate the expression of miR-10a in the different FAB subtype of acute myeloid leukemia (AML) and its relationship with drug resistance. Methods: Forty de novo patients with AML, 16 patients with non-malignant hematologic disease and three AML cell lines HL-60, U937 and HL-60/ADR were enrolled in this study, the MiR-10a expression in bone marrow mononuclear cells of above-mentioned patients and 3 AML cell lines was detected by TaqMan RT-PCR. The correlation of miR-10a with clinicopathological factors of AML patients was analyzed. Results: The miR-10a expression level in HL-60 cell line was higher than that in U937 cell line (P = 0.039). And its expression level in de novo AML patients was higher than that in patients with non-malignant hematologic disease (P < 0.01). FAB-AML-M3 patients exhibited higher expression of miR-10a than that in M1, M2 and M4 (P < 0.05); HL-60/ADR cell line showed higher miR-10a expression than that in HL-60 cell line (P < 0.01) . Except M3, the patients without CR (non-CR) after the first cycle of chemotherapy showed a higher level of miR-10a as compared with CR patients (P < 0.01). Conclusion: The high expression of miR-10a may be closely related to over-proliferation of promyelocyte and drug resistance of acute myeloid leukemia cells, except M3.
Databáze:	MEDLINE
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