| Autor: |
Hong ME; Department of Chemical Engineering, Sungkyunkwan University, Suwon, 440-746, South Korea., Hwang SK, Chang WS, Kim BW, Lee J, Sim SJ |
| Jazyk: |
angličtina |
| Zdroj: |
Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2015 Jun; Vol. 99 (12), pp. 5203-15. Date of Electronic Publication: 2015 Feb 17. |
| DOI: |
10.1007/s00253-015-6440-5 |
| Abstrakt: |
High temperatures (30-36 °C) inhibited astaxanthin accumulation in Haematococcus pluvialis under photoautotrophic conditions. The depression of carotenogenesis was primarily attributed to excess intracellular less reactive oxygen species (LROS; O2 (-) and H2O2) levels generated under high temperature conditions. Here, we show that the heat stress-driven inefficient astaxanthin production was improved by accelerating the iron-catalyzed Haber-Weiss reaction to convert LROS into more reactive oxygen species (MROS; O2 and OH·), thereby facilitating lipid peroxidation. As a result, during 18 days of photoautotrophic induction, the astaxanthin concentration of cells cultured in high temperatures in the presence of iron (450 μM) was dramatically increased by 75 % (30 °C) and 133 % (36 °C) compared to that of cells exposed to heat stress alone. The heat stress-driven Haber-Weiss reaction will be useful for economically producing astaxanthin by reducing energy cost and enhancing photoautotrophic astaxanthin production, particularly outdoors utilizing natural solar radiation including heat and light for photo-induction of H. pluvialis. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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