A PCR-RFLP method for the simultaneous differentiation of three Entamoeba species.

Autor: Fontecha GA; Microbiology Research Institute, Microbiology School, National Autonomous University of Honduras (UNAH), Ciudad Universitaria, J1, 4th fl., Tegucigalpa, Honduras. Electronic address: gustavo.fontecha@unah.edu.hn., García K; Microbiology Research Institute, Microbiology School, National Autonomous University of Honduras (UNAH), Ciudad Universitaria, J1, 4th fl., Tegucigalpa, Honduras., Rueda MM; Microbiology Research Institute, Microbiology School, National Autonomous University of Honduras (UNAH), Ciudad Universitaria, J1, 4th fl., Tegucigalpa, Honduras., Sosa-Ochoa W; Microbiology Research Institute, Microbiology School, National Autonomous University of Honduras (UNAH), Ciudad Universitaria, J1, 4th fl., Tegucigalpa, Honduras., Sánchez AL; Microbiology Research Institute, Microbiology School, National Autonomous University of Honduras (UNAH), Ciudad Universitaria, J1, 4th fl., Tegucigalpa, Honduras; Department of Health Sciences, Brock University, St. Catharines, ON, Canada., Leiva B; Microbiology and Parasitology Department, Faculty of Medical Sciences, Infectious Diseases Research Center, UNAN-León, León, Nicaragua.
Jazyk: angličtina
Zdroj: Experimental parasitology [Exp Parasitol] 2015 Apr-May; Vol. 151-152, pp. 80-3. Date of Electronic Publication: 2015 Feb 10.
DOI: 10.1016/j.exppara.2015.02.003
Abstrakt: Amoebiasis caused by Entamoeba histolytica continues to be one of the most common parasitic diseases in the developing world. Despite its relevance, due to the lack of accurate diagnostic methods, the true clinical and public health importance of this parasite remains uncertain. The aim of this study was to develop a new diagnostic tool to differentiate E.histolytica from the morphologically undistinguishable E.dispar and E.moshkovskii. We developed a specific, fast and simple PCR-RFLP method that was able to accurately differentiate experimentally-obtained restriction patterns from the three Entamoeba species. This new method could prove useful for clinical and epidemiological purposes.
(Copyright © 2015 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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