Thiol-responsive gemini poly(ethylene glycol)-poly(lactide) with a cystine disulfide spacer as an intracellular drug delivery nanocarrier.

Autor: Kim HC; Nano & Bio Research Division, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu 711-873, Republic of Korea. Electronic address: kimhc@dgist.ac.kr., Kim E; Nano & Bio Research Division, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu 711-873, Republic of Korea. Electronic address: ejkim@dgist.ac.kr., Ha TL; Nano & Bio Research Division, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu 711-873, Republic of Korea., Jeong SW; Nano & Bio Research Division, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu 711-873, Republic of Korea., Lee SG; Nano & Bio Research Division, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu 711-873, Republic of Korea., Lee SJ; Nano & Bio Research Division, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu 711-873, Republic of Korea., Lee B; Nano & Bio Research Division, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu 711-873, Republic of Korea.
Jazyk: angličtina
Zdroj: Colloids and surfaces. B, Biointerfaces [Colloids Surf B Biointerfaces] 2015 Mar 01; Vol. 127, pp. 206-12. Date of Electronic Publication: 2015 Jan 31.
DOI: 10.1016/j.colsurfb.2015.01.039
Abstrakt: Thiol-responsive gemini micelles consisting of hydrophilic poly(ethylene glycol) (PEG) blocks and hydrophobic polylactide (PLA) blocks with a cystine disulfide spacer were reported as effective intracellular nanocarriers of drugs. In the presence of cellular glutathione (GSH) as a reducing agent, gemini micelles gradually destabilize into monomeric micelles through cleavage of the cystine linkage. This destabilization of the gemini micelles changed their size distribution, with the appearance of small aggregates, and led to the enhanced release of encapsulated doxorubicin (DOX). The results obtained from cell culture via confocal laser scanning microscopy (CLSM) for cellular uptake, as well as cell viability measurements for anticancer efficacy suggest the potential of disulfide-based gemini polymeric micelles as controlled drug delivery carriers.
(Copyright © 2015 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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