| Autor: |
Siti W; Centre for Biomimetic Sensor Science, School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Drive 637553, Singapore. MNallani@ntu.edu.sg., Khan AK, de Hoog HP, Liedberg B, Nallani M |
| Jazyk: |
angličtina |
| Zdroj: |
Organic & biomolecular chemistry [Org Biomol Chem] 2015 Mar 21; Vol. 13 (11), pp. 3202-6. |
| DOI: |
10.1039/c4ob02025a |
| Abstrakt: |
Bio-orthogonal chemistry has been widely used for conjugation of polymer molecules to proteins. Here, we demonstrate the conjugation of polyethylene glycol (PEG) to bovine beta-lactoglobulin (BLG) by photo-induced cyclo-addition of tetrazole-appended PEG and allyl-modified BLG. During the course of the investigation, a significant side-reaction was found to occur for the conjugation of PEG-tetrazole to native BLG. Further exploration of the underlying chemistry reveals that the presence of a tryptophan residue is sufficient for conjugation of tetrazole-modified molecules. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
