| Autor: |
Sanz-Fernández R; Department of Otolaryngology, University Hospital of Getafe, Autovía de Toledo, km 12,500, 28905, Getafe, Madrid, Spain. rsanzf@salud.madrid.org., Sánchez-Rodriguez C; Fundación para la Investigación Biomédica, University Hospital of Getafe, Getafe, Madrid, Spain., Granizo JJ; Clinical Epidemiology Unit, Hospital Infanta Cristina, Parla, Madrid, Spain., Durio-Calero E; Department of Otolaryngology, University Hospital of Getafe, Autovía de Toledo, km 12,500, 28905, Getafe, Madrid, Spain., Martín-Sanz E; Department of Otolaryngology, University Hospital of Getafe, Autovía de Toledo, km 12,500, 28905, Getafe, Madrid, Spain. |
| Abstrakt: |
Aging causes histological, electrophysiological and molecular changes in the cochlea. The free radical theory of aging, has obtained consensus, and the mitochondrion is reported to play a key role in aging as a major source of reactive oxygen species. In the last years, there has been a significant increase in the interest in polyphenols because of the antioxidant properties and their role in the prevention of various diseases associated with oxidative stress, including aging. The aim of this study was to evaluate the preventive effect of different polyphenols on ARHL with auditory-evoked potentials. 100 Healthy female Sprague-Dawley (SD) rats were used for this study. Five groups were created based on the age of the rats, in months: 3, 6, 12, 18 and 24 months old. Two additional groups were created based on the treatment received. In the control group, 50 animals were assigned to no treatment. In the treated group, 50 animals were given a vehicle mixture of polyphenols for the half of the life before euthanization. Nine frequencies were tested (0.5-16 kHz) with ASSR and tone-burst ABR, performed on all of the rats prior to sacrifice. 100-μs auditory clicks ABRs were also recorded. A significant decrease in the audition was detected with ABR and ASSR in both treated and non-treated groups, as the different groups became older. This deterioration was more accurately measured at acute frequencies. Significantly lower thresholds were observed in the treated rats in the 6, 12 and 18-month-old group in the treated rats compared with the control group. All of the thresholds elicited using the ASSR technique were lower than the thresholds obtained using the ABR, regardless of the stimulus type. The present study demonstrated the benefits of the polyphenols, which generated a significant protection against ARHL, with significantly improved ASSR and tone-burst ABR auditory thresholds in rats receiving treatment with polyphenols. |
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