Daily variations in the expression of miR-16 and miR-181a in human leukocytes.

Autor: Figueredo Dde S; Laboratory of Molecular Chronobiology, Universidade Federal de Alagoas, Campus Arapiraca, Alagoas, Brazil., Gitaí DL; Laboratory of Cellular and Molecular Biology, Universidade Federal de Alagoas, Maceió, Alagoas, Brazil., Andrade TG; Laboratory of Molecular Chronobiology, Universidade Federal de Alagoas, Campus Arapiraca, Alagoas, Brazil. Electronic address: deandrade.tiago@pq.cnpq.br.
Jazyk: angličtina
Zdroj: Blood cells, molecules & diseases [Blood Cells Mol Dis] 2015 Apr; Vol. 54 (4), pp. 364-8. Date of Electronic Publication: 2015 Jan 17.
DOI: 10.1016/j.bcmd.2015.01.004
Abstrakt: Circadian rhythms are controlled by a molecular mechanism that is organized in transcriptional and translational feedback loops of gene expression. Recent studies have been demonstrating the involvement of microRNAs (miRs) in post-transcriptional/translational control of circadian rhythms. In the present study we aimed to analyze the daily variations of miR-16 and miR-181a expression in human leukocytes. These miRs were independently associated with hematopoiesis and circadian rhythms in previous studies using experimental models. Peripheral blood from 6 subjects was sampled in a 24 hour period for expression analysis using quantitative real-time PCR (RT-qPCR). Initially, we evaluated the expression stability of RNU6-2, RNU1A-1, RNU5A-1, SNORD-25, SCARNA-17 and SNORA-73A as candidate genes for normalization of RT-qPCR data. The combination of the four most stable genes (SNORA-73A/SCARNA-17/SNORD-25/RNU6-2) was indicated to provide a better normalization of miRs expressions. The results show a daily variation of miR-181a and miR-16 expression in human leukocytes, suggesting a potential participation of these genes in the modulation of the circadian rhythms present in blood cells.
(Copyright © 2015 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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