Matrix-assisted laser desorption ionization-time of flight mass spectrometry for differentiation of the dimorphic fungal species Paracoccidioides brasiliensis and Paracoccidioides lutzii.

Autor:	Nobrega de Almeida J Jr; Central Laboratory Division-LIM03, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil Laboratory of Medical Mycology-LIM53, Hospital das Clínicas FMUSP, Instituto de Medicina Tropical, Universidade de São Paulo, São Paulo, Brazil jnaj99@gmail.com., Del Negro GM; Laboratory of Medical Mycology-LIM53, Hospital das Clínicas FMUSP, Instituto de Medicina Tropical, Universidade de São Paulo, São Paulo, Brazil., Grenfell RC; Department of Biophysics, Escola Paulista Medicina, Universidade Federal de São Paulo, São Paulo, Brazil., Vidal MS; Laboratory of Medical Mycology-LIM53, Hospital das Clínicas FMUSP, Instituto de Medicina Tropical, Universidade de São Paulo, São Paulo, Brazil., Thomaz DY; Laboratory of Medical Mycology-LIM53, Hospital das Clínicas FMUSP, Instituto de Medicina Tropical, Universidade de São Paulo, São Paulo, Brazil., de Figueiredo DS; Laboratory of Medical Mycology-LIM53, Hospital das Clínicas FMUSP, Instituto de Medicina Tropical, Universidade de São Paulo, São Paulo, Brazil., Bagagli E; Department of Microbiology and Immunology, Instituto de Biociências de Botucatu, Universidade Estadual Paulista Júlio de Mesquita Filho, Botucatu, Brazil., Juliano L; Department of Biophysics, Escola Paulista Medicina, Universidade Federal de São Paulo, São Paulo, Brazil., Benard G; Laboratory of Medical Mycology-LIM53, Hospital das Clínicas FMUSP, Instituto de Medicina Tropical, Universidade de São Paulo, São Paulo, Brazil.
Jazyk:	angličtina
Zdroj:	Journal of clinical microbiology [J Clin Microbiol] 2015 Apr; Vol. 53 (4), pp. 1383-6. Date of Electronic Publication: 2015 Jan 28.
DOI:	10.1128/JCM.02847-14
Abstrakt:	Isolates of Paracoccidioides brasiliensis and Paracoccidioides lutzii, previously characterized by molecular techniques, were identified for the first time by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). All isolates were correctly identified, with log score values of >2.0. Thus, MALDI-TOF MS is a new tool for differentiating species of the genus Paracoccidioides. (Copyright © 2015, American Society for Microbiology. All Rights Reserved.)
Databáze:	MEDLINE
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