Effect of glutamine supplementation on left colon healing in rats with extrahepatic biliary obstruction.
| Autor: | Alecrim HM; School of Medicine, Brasilia University, Brazil., Duarte SA; School of Medicine, Brasilia University, Brazil., Amaral ME; School of Medicine, UnB, Brasília, DF, Brazil., Diógenis F; School of Medicine, UnB, Brasília, DF, Brazil., Carneiro FP; School of Medicine, UnB, Brasília, DF, Brazil., Sousa JB; School of Medicine, UnB, Brasília, DF, Brazil. |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | Acta cirurgica brasileira [Acta Cir Bras] 2015 Jan; Vol. 30 (1), pp. 73-9. |
| DOI: | 10.1590/S0102-86502015001000010 |
| Abstrakt: | Purpose: To evaluate the influence of glutamine and obstructive jaundice on left colon healing in rats. Methods: Sixteen male rats were allocated across four groups: LG--Common bile duct ligation followed by colotomy and bowel suture on postoperative day 7. Supplementation with glutamine 2% from day 4 after duct ligation until euthanasia. L--Common bile duct ligation followed by colotomy and bowel suture on postoperative day 7. No glutamine supplementation. M--Common bile duct manipulation followed by colotomy and bowel suture on postoperative day 7. No glutamine supplementation. MG--Common bile duct manipulation followed by colotomy and bowel suture on postoperative day 7. Supplementation with glutamine 2% from day 4 after duct manipulation until euthanasia. On the day of euthanasia, bursting pressure of the sutured bowel segment was measured and samples were collected for histopathological analysis. Results: There were no differences in bursting pressure among groups : LG vs. M (110 ± 28 vs. 173 ± 12; p = 0.08). Groups L and MG were not different from group M (156 ± 12 and 118 ± 22. Glutamine supplementation was associated with less edema, polymorphonuclear lymphocyte infiltration, bacterial colonies, and abscess formation, as well as with increased collagen formation. Conclusion: Obstructive jaundice had no negative effect and glutamine supplementation had no positive effect on colonic scar strength in rats. |
| Databáze: | MEDLINE |
| Externí odkaz: |
|