| Autor: |
Burke KA; University of Rochester, Department of Biomedical Engineering, 207 Robert B. Goergen Hall, P.O. Box 270168, Rochester, New York 14627, United States., Dawes RP; University of Rochester, Department of Neurobiology and Anatomy, 601 Elmwood Avenue, Rochester, New York 14642, United States., Cheema MK; State University of New York at Stony Brook, Department of Biomedical Engineering, Stony Brook, New York 11790, United States., Van Hove A; University of Rochester, Department of Biomedical Engineering, 207 Robert B. Goergen Hall, P.O. Box 270168, Rochester, New York 14627, United States., Benoit DS; University of Rochester, Department of Biomedical Engineering, 207 Robert B. Goergen Hall, P.O. Box 270168, Rochester, New York 14627, United StatesdUniversity of Rochester, Department of Biomedical Genetics, 601 Elmwood Avenue, Rochester, New York 14642., Perry SW; University of Rochester, Department of Biomedical Engineering, 207 Robert B. Goergen Hall, P.O. Box 270168, Rochester, New York 14627, United States., Brown E; University of Rochester, Department of Biomedical Engineering, 207 Robert B. Goergen Hall, P.O. Box 270168, Rochester, New York 14627, United StatesbUniversity of Rochester, Department of Neurobiology and Anatomy, 601 Elmwood Avenue, Rochester, New York 1. |
| Abstrakt: |
Second-harmonic generation (SHG) allows for the analysis of tumor collagen structural changes throughout metastatic progression. SHG directionality, measured through the ratio of the forward-propagating to backward-propagating signal (F/B ratio), is affected by collagen fibril diameter, spacing, and disorder of fibril packing within a fiber. As tumors progress, these parameters evolve, producing concurrent changes in F/B. It has been recently shown that the F/B of highly metastatic invasive ductal carcinoma (IDC) breast tumors is significantly different from less metastatic tumors. This suggests a possible relationship between the microstructure of collagen, as measured by the F/B, and the ability of tumor cells to locomote through that collagen. Utilizing in vitro collagen gels of different F/B ratios, we explored the relationship between collagen microstructure and motility of tumor cells in a “clean” environment, free of the myriad cells, and signals found in in vivo. We found a significant relationship between F/B and the total distance traveled by the tumor cell, as well as both the average and maximum velocities of the cells. Consequently, one possible mechanism underlying the observed relationship between tumor F/B and metastatic output in IDC patient samples is a direct influence of collagen structure on tumor cell motility. |
