The cerebral cavernous malformation pathway controls cardiac development via regulation of endocardial MEKK3 signaling and KLF expression.

Autor:	Zhou Z; Department of Medicine and Cardiovascular Institute, University of Pennsylvania, 3400 Civic Center Boulevard, Philadelphia, PA 19104, USA., Rawnsley DR; Department of Medicine and Cardiovascular Institute, University of Pennsylvania, 3400 Civic Center Boulevard, Philadelphia, PA 19104, USA., Goddard LM; Department of Medicine and Cardiovascular Institute, University of Pennsylvania, 3400 Civic Center Boulevard, Philadelphia, PA 19104, USA., Pan W; Department of Medicine and Cardiovascular Institute, University of Pennsylvania, 3400 Civic Center Boulevard, Philadelphia, PA 19104, USA., Cao XJ; Department of Biochemistry and Biophysics, University of Pennsylvania, 3400 Civic Center Boulevard, Philadelphia, PA 19104, USA., Jakus Z; Department of Medicine and Cardiovascular Institute, University of Pennsylvania, 3400 Civic Center Boulevard, Philadelphia, PA 19104, USA., Zheng H; Department of Medicine and Cardiovascular Institute, University of Pennsylvania, 3400 Civic Center Boulevard, Philadelphia, PA 19104, USA., Yang J; Department of Medicine and Cardiovascular Institute, University of Pennsylvania, 3400 Civic Center Boulevard, Philadelphia, PA 19104, USA., Arthur JS; Division of Cell Signaling and Immunology, University of Dundee, Dundee DD1 5EH, UK., Whitehead KJ; Division of Cardiovascular Medicine and the Program in Molecular Medicine, University of Utah, Salt Lake City, UT 84112, USA., Li D; Division of Cardiovascular Medicine and the Program in Molecular Medicine, University of Utah, Salt Lake City, UT 84112, USA; The Key Laboratory for Human Disease Gene Study of Sichuan Province, Institute of Laboratory Medicine, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu, Sichuan 610072, China., Zhou B; Department of Genetics, Pediatric, and Medicine (Cardiology) and Wilf Cardiovascular Research Institute, Albert Einstein College of Medicine of Yeshiva University, 1301 Morris Park Avenue, Bronx, NY 10461, USA., Garcia BA; Department of Biochemistry and Biophysics, University of Pennsylvania, 3400 Civic Center Boulevard, Philadelphia, PA 19104, USA., Zheng X; Department of Medicine and Cardiovascular Institute, University of Pennsylvania, 3400 Civic Center Boulevard, Philadelphia, PA 19104, USA; Lab of Cardiovascular Signaling, Centenary Institute, Sydney NSW 2050, Australia. Electronic address: x.zheng@centenary.org.au., Kahn ML; Department of Medicine and Cardiovascular Institute, University of Pennsylvania, 3400 Civic Center Boulevard, Philadelphia, PA 19104, USA. Electronic address: markkahn@mail.med.upenn.edu.
Jazyk:	angličtina
Zdroj:	Developmental cell [Dev Cell] 2015 Jan 26; Vol. 32 (2), pp. 168-80.
DOI:	10.1016/j.devcel.2014.12.009
Abstrakt:	The cerebral cavernous malformation (CCM) pathway is required in endothelial cells for normal cardiovascular development and to prevent postnatal vascular malformations, but its molecular effectors are not well defined. Here we show that loss of CCM signaling in endocardial cells results in mid-gestation heart failure associated with premature degradation of cardiac jelly. CCM deficiency dramatically alters endocardial and endothelial gene expression, including increased expression of the Klf2 and Klf4 transcription factors and the Adamts4 and Adamts5 proteases that degrade cardiac jelly. These changes in gene expression result from increased activity of MEKK3, a mitogen-activated protein kinase that binds CCM2 in endothelial cells. MEKK3 is both necessary and sufficient for expression of these genes, and partial loss of MEKK3 rescues cardiac defects in CCM-deficient embryos. These findings reveal a molecular mechanism by which CCM signaling controls endothelial gene expression during cardiovascular development that may also underlie CCM formation. (Copyright © 2015 Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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