Calotropis procera extract induces apoptosis and cell cycle arrest at G2/M phase in human skin melanoma (SK-MEL-2) cells.

Autor: Joshi AL; a Deogiri College, Centre for Advanced Life Sciences , Aurangabad , Maharashtra 43100 , India., Roham PH; a Deogiri College, Centre for Advanced Life Sciences , Aurangabad , Maharashtra 43100 , India., Mhaske R; a Deogiri College, Centre for Advanced Life Sciences , Aurangabad , Maharashtra 43100 , India., Jadhav M; a Deogiri College, Centre for Advanced Life Sciences , Aurangabad , Maharashtra 43100 , India., Krishnadas K; a Deogiri College, Centre for Advanced Life Sciences , Aurangabad , Maharashtra 43100 , India., Kharat A; b Modern College of Pharmacy , Moshi, Pune 412105 , India., Hardikar B; c KET's V.G. Vaze College , Mulund, Mumbai 400081 , India., Kharat KR; a Deogiri College, Centre for Advanced Life Sciences , Aurangabad , Maharashtra 43100 , India.
Jazyk: angličtina
Zdroj: Natural product research [Nat Prod Res] 2015; Vol. 29 (23), pp. 2261-4. Date of Electronic Publication: 2015 Jan 27.
DOI: 10.1080/14786419.2014.1001386
Abstrakt: Calotropis procera (family: Asclepiadaceae) contains cardiac glycosides which are cytotoxic to cancer cells. The extracts of C. procera have been reported to be cytotoxic to many cancer cell lines and this is the first report against the human skin melanoma cells (SK-MEL-2). The SK-MEL-2 cells treated with C. procera methanolic extract (CPME) were analysed for growth inhibition and apoptosis. The exposure of phosphatidylserine in apoptotic SK-MEL-2 was analysed by using the Annexin-V FITC flow cytometry method. In CPME-treated SK-MEL-2 cells, 19.6% of apoptotic and 58.3% dead cells were observed. The 15.97% and 15.85% of early apoptotic cells were found at 20 μg/mL of the ouabain and paclitaxel, respectively. Active caspases, nuclear degradation confirmed apoptotic SK-MEL-2 cells in time- and dose-dependent manner. The cell cycle analysis shows that CPME treated cells halt at G2/M phase. Significant cytotoxic activity of CPME against SK-MEL-2 may be attributed to its high cardenolide content.
Databáze: MEDLINE
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