Recombinant interleukin-1 receptor antagonist conjugated to superparamagnetic iron oxide nanoparticles for theranostic targeting of experimental glioblastoma.

Autor:	Shevtsov MA; Institute of Cytology of the Russian Academy of Sciences (RAS), St. Petersburg, Russia; A.L. Polenov Russian Research Scientific Institute of Neurosurgery, St. Petersburg, Russia. Electronic address: shevtsov-max@mail.ru., Nikolaev BP; Research Institute of Highly Pure Biopreparations, St. Petersburg, Russia., Yakovleva LY; Research Institute of Highly Pure Biopreparations, St. Petersburg, Russia., Dobrodumov AV; Institute of Macromolecular Compounds of the Russian Academy of Sciences (RAS), St. Petersburg, Russia., Zhakhov AV; Research Institute of Highly Pure Biopreparations, St. Petersburg, Russia., Mikhrina AL; I.M. Sechenov Institute of Evolutionary Physiology and Biochemistry of the Russian Academy of Sciences (RAS), St. Petersburg, Russia., Pitkin E; Wharton School, University of Pennsylvania, Philadelphia, PA, USA., Parr MA; V.F. Fock Institute of Physics, St. Petersburg State University, St. Petersburg, Russia., Rolich VI; V.F. Fock Institute of Physics, St. Petersburg State University, St. Petersburg, Russia., Simbircev AS; Research Institute of Highly Pure Biopreparations, St. Petersburg, Russia., Ischenko AM; Research Institute of Highly Pure Biopreparations, St. Petersburg, Russia.
Jazyk:	angličtina
Zdroj:	Neoplasia (New York, N.Y.) [Neoplasia] 2015 Jan; Vol. 17 (1), pp. 32-42.
DOI:	10.1016/j.neo.2014.11.001
Abstrakt:	Cerebral edema commonly accompanies brain tumors and contributes to neurologic symptoms. The role of the interleukin-1 receptor antagonist conjugated to superparamagnetic iron oxide nanoparticles (SPION-IL-1Ra) was assessed to analyze its anti-edemal effect and its possible application as a negative contrast enhancing agent for magnetic resonance imaging (MRI). Rats with intracranial C6 glioma were intravenously administered at various concentrations of IL-1Ra or SPION-IL-1Ra. Brain peritumoral edema following treatment with receptor antagonist was assessed with high-field MRI. IL-1Ra administered at later stages of tumor progression significantly reduced peritumoral edema (as measured by MRI) and prolonged two-fold the life span of comorbid animals in a dose-dependent manner in comparison to control and corticosteroid-treated animals (P < .001). Synthesized SPION-IL-1Ra conjugates had the properties of negative contrast agent with high coefficients of relaxation efficiency. In vitro studies of SPION-IL-1Ra nanoparticles demonstrated high intracellular incorporation and absence of toxic influence on C6 cells and lymphocyte viability and proliferation. Retention of the nanoparticles in the tumor resulted in enhanced hypotensive T2-weighted images of glioma, proving the application of the conjugates as negative magnetic resonance contrast agents. Moreover, nanoparticles reduced the peritumoral edema confirming the therapeutic potency of synthesized conjugates. SPION-IL-1Ra nanoparticles have an anti-edemal effect when administered through a clinically relevant route in animals with glioma. The SPION-IL-1Ra could be a candidate for theranostic approach in neuro-oncology both for diagnosis of brain tumors and management of peritumoral edema. (Copyright © 2014 Neoplasia Press, Inc. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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