A silk-based encapsulation platform for pancreatic islet transplantation improves islet function in vivo.
| Autor: | Hamilton DC; Department of Pathology, Stanford University School of Medicine, CA, USA., Shih HH; Department of Pathology, Stanford University School of Medicine, CA, USA., Schubert RA; Department of Pathology, Stanford University School of Medicine, CA, USA., Michie SA; Department of Pathology, Stanford University School of Medicine, CA, USA., Staats PN; Department of Pathology, University of Maryland School of Medicine, Baltimore, MD, USA., Kaplan DL; Department of Bioengineering, Tufts University, Medford, MA, USA., Fontaine MJ; Department of Pathology, Stanford University School of Medicine, CA, USA.; Department of Pathology, University of Maryland School of Medicine, Baltimore, MD, USA. |
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| Jazyk: | angličtina |
| Zdroj: | Journal of tissue engineering and regenerative medicine [J Tissue Eng Regen Med] 2017 Mar; Vol. 11 (3), pp. 887-895. Date of Electronic Publication: 2015 Jan 26. |
| DOI: | 10.1002/term.1990 |
| Abstrakt: | The success of pancreatic islet (PI) transplantation is challenged by PI functional damage during the peritransplantation period. A silk-based encapsulation platform including mesenchymal stromal cells (MSCs) was evaluated for islet cell delivery in vivo. Islet equivalents (IEQs) were transplanted into the epididymal fat pads of mice with streptozotocin-induced diabetes. Three PI combinations were tested: (A) co-encapsulated in silk with MSCs; (b) encapsulated in silk alone; or (c) pelleted. Blood glucose levels were monitored and intraperitoneal glucose tolerance test (IPGTT) was performed upon return to euglycaemia. Grafts were removed for histology and cytokine content analysis. Mice with PI grafts in silk showed a prompt return to euglycaemia. IPGTT was significantly improved with PI in silk with MSCs, compared to PI in silk alone or pelleted. Both Th (Copyright © 2015 John Wiley & Sons, Ltd.) |
| Databáze: | MEDLINE |
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