Development of the follicular basement membrane during human gametogenesis and early folliculogenesis.

Autor: Heeren AM; Department of Anatomy and Embryology, Leiden University Medical Center, Einthovenweg 20, 2333, Leiden, ZC, The Netherlands. marijneheeren@gmail.com.; Department of Gynaecology, Leiden University Medical Center, Albinusdreef 2, 2300, Leiden, RC, The Netherlands. marijneheeren@gmail.com.; Current address: Department of Obstetrics and Gynaecology, VU University Medical Center, De Boelelaan 1118, 1081, Amsterdam, HZ, The Netherlands. marijneheeren@gmail.com., van Iperen L; Department of Anatomy and Embryology, Leiden University Medical Center, Einthovenweg 20, 2333, Leiden, ZC, The Netherlands. L.van_Iperen@lumc.nl., Klootwijk DB; Department of Anatomy and Embryology, Leiden University Medical Center, Einthovenweg 20, 2333, Leiden, ZC, The Netherlands. danielle.k90@gmail.com., de Melo Bernardo A; Department of Anatomy and Embryology, Leiden University Medical Center, Einthovenweg 20, 2333, Leiden, ZC, The Netherlands. A.B.de_Melo_Bernardo@lumc.nl., Roost MS; Department of Anatomy and Embryology, Leiden University Medical Center, Einthovenweg 20, 2333, Leiden, ZC, The Netherlands. M.S.Roost@lumc.nl., Gomes Fernandes MM; Department of Anatomy and Embryology, Leiden University Medical Center, Einthovenweg 20, 2333, Leiden, ZC, The Netherlands. M.M.Gomes_Fernandes@lumc.nl., Louwe LA; Department of Gynaecology, Leiden University Medical Center, Albinusdreef 2, 2300, Leiden, RC, The Netherlands. L.A.Louwe@lumc.nl., Hilders CG; Department of Gynaecology, Reinier de Graaf Hospital, Reinier de Graaf 3-11, 2625, Delft, AD, The Netherlands. Hilders@rdgg.nl., Helmerhorst FM; Department of Gynaecology, Leiden University Medical Center, Albinusdreef 2, 2300, Leiden, RC, The Netherlands. F.M.Helmerhorst@lumc.nl., van der Westerlaken LA; Department of Gynaecology, Leiden University Medical Center, Albinusdreef 2, 2300, Leiden, RC, The Netherlands. L.A.J.van_der_Westerlaken@lumc.nl., Chuva de Sousa Lopes SM; Department of Anatomy and Embryology, Leiden University Medical Center, Einthovenweg 20, 2333, Leiden, ZC, The Netherlands. lopes@lumc.nl.; Department for Reproductive Medicine, Ghent University Hospital, De Pintelaan 185, 9000, Ghent, Belgium. lopes@lumc.nl.
Jazyk: angličtina
Zdroj: BMC developmental biology [BMC Dev Biol] 2015 Jan 21; Vol. 15, pp. 4. Date of Electronic Publication: 2015 Jan 21.
DOI: 10.1186/s12861-015-0054-0
Abstrakt: Background: In society, there is a clear need to improve the success rate of techniques to restore fertility. Therefore a deeper knowledge of the dynamics of the complex molecular environment that regulates human gametogenesis and (early) folliculogenesis in vivo is necessary. Here, we have studied these processes focusing on the formation of the follicular basement membrane (BM) in vivo.
Results: The distribution of the main components of the extracellular matrix (ECM) collagen IV, laminin and fibronectin by week 10 of gestation (W10) in the ovarian cortex revealed the existence of ovarian cords and of a distinct mesenchymal compartment, resembling the organization in the male gonads. By W17, the first primordial follicles were assembled individually in that (cortical) mesenchymal compartment and were already encapsulated by a BM of collagen IV and laminin, but not fibronectin. In adults, in the primary and secondary follicles, collagen IV, laminin and to a lesser extent fibronectin were prominent in the follicular BM.
Conclusions: The ECM-molecular niche compartimentalizes the female gonads from the time of germ cell colonization until adulthood. This knowledge may contribute to improve methods to recreate the environment needed for successful folliculogenesis in vitro and that would benefit a large number of infertility patients.
Databáze: MEDLINE
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