| Autor: |
Korneyev AYa; Institute of Pharmacology, Acad. Med. Sci. U.S.S.R., Moscow., Meliksetyan MB |
| Jazyk: |
angličtina |
| Zdroj: |
European journal of pharmacology [Eur J Pharmacol] 1989 Dec 07; Vol. 173 (2-3), pp. 201-5. |
| DOI: |
10.1016/0014-2999(89)90521-9 |
| Abstrakt: |
Specific, saturable and reversible binding of [3H]beta + CCE ([3H]beta-carboline-3-carboxylic acid ethyl ester) in buffer containing 20 microM AgNO3 and 10 microM diazepam was detected in rat brain membranes. The binding of [3H]beta CCE to non-benzodiazepine binding sites is Ag+ (Cu)-dependent, stimulated by NaCl and ascorbic acid and inhibited by dithiothreitol. The concentration of non-benzodiazepine [3H]beta CCE binding sites (Bmax) determined in the brain membranes was 1180 +/- 320 pmol/g tissue, and Kd = 77 +/- 19 nM. [3H]beta CCE bound to benzodiazepine receptors in the same membranes with Bmax = 81 +/- 9 pmol/g tissue and Kd = 3.2 +/- 0.4 nM. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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